Expression dynamics of CXCL 12 and CXCR 4 during the progression of mycosis fungoides
| Autor: | Masanobu Kitagawa, Iichiroh Onishi, K. Miura, Shinya Abe, Y. Sawada, T. Tanizawa, R.N. Daggett, Morito Kurata |
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| Rok vydání: | 2014 |
| Předmět: |
Adult
Male Receptors CXCR4 Chemokine Pathology medicine.medical_specialty Skin Neoplasms T-Lymphocytes Cell Dermatology medicine.disease_cause CXCR4 Mycosis Fungoides medicine Humans RNA Messenger Stage (cooking) Receptor Aged Skin Aged 80 and over Mycosis fungoides biology Reverse Transcriptase Polymerase Chain Reaction Macrophages Middle Aged medicine.disease Immunohistochemistry Chemokine CXCL12 medicine.anatomical_structure Case-Control Studies Disease Progression biology.protein Female Carcinogenesis |
| Zdroj: | British Journal of Dermatology. 171:722-731 |
| ISSN: | 1365-2133 0007-0963 |
| Popis: | Summary Background Mycosis fungoides (MF) classically presents from patch stage to plaque stage over a number of years and finally progresses to tumour stage with nodal or visceral involvement. The mechanism of progression remains incompletely elucidated. Chemokines and their receptors are known to be involved in disease mechanisms, with CXCL12 and CXCR4 playing a critical role in carcinogenesis, invasion and cancer cell migration in various carcinomas. Objectives To investigate the expression of CXCL12 and CXCR4 in different cutaneous stages of MF. Methods Formalin-fixed, paraffin-embedded skin samples from 40 patients with MF (21 patch stage, 10 plaque stage, nine tumour stage) and 30 non-neoplastic control skin samples were analysed. CXCL12 and CXCR4 were assessed by quantitative reverse-transcription polymerase chain reaction and immunohistochemical staining. Results The expression level of mRNA for CXCL12 in plaque-stage MF was significantly higher than in control skin (P = 0·0035), or patch-stage (P = 0·0108) or tumour-stage disease (P = 0·0089). The CXCR4 mRNA expression level in plaque-stage disease was significantly higher than in control skin (P = 0·0090) or patch-stage disease (P = 0·0387). CXCL12- and CXCR4-positive cell rates in patch-stage and plaque-stage MF were significantly higher than those in control skin (P |
| Databáze: | OpenAIRE |
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