Role of E-Cadherin in Epithelial Architecture Maintenance

Autor: Nathalie B. Vicente, M. F. Izaguirre, Javier Adur, Javier Diaz-Zamboni, Delfina Larrea, Victor Hugo Casco, C. D. Galetto
Rok vydání: 2010
Předmět:
Zdroj: Cell Communication & Adhesion. 17:1-12
ISSN: 1543-5180
1541-9061
DOI: 10.3109/15419061003686938
Popis: Morphogenesis and architecture of a developing epithelium is controlled by both cell shape and contacts, mediated by spatially and temporally regulated cell adhesion molecules. The authors study if E-cadherin functions as a key factor of epithelial adhesion and epidermal architecture in vivo. They apply whole-mount digital deconvolution microscopy to evaluate three-dimensional (3D) E-cadherin expression during skin morphogenesis of Rhinella arenarum and in a cell adhesion alteration model. Results show morphogenetic changes in the 3D E-cadherin spatiotemporal expression pattern correlated with the increase of E-cadherin and in the number of cells with hexagonal geometry. Alterations in junction-protein phosphorylation showed drastic loss of E-cadherin and beta-catenin in cell-cell contacts and the increase of cytoplasm and nuclear beta-catenin in epidermis, suggesting the activation of the beta-catenin signal pathway. Surprisingly, no changes in cell shape and skin architecture were registered, suggesting that epidermal E-cadherin appears to be involved in signaling rather than cell contact maintenance in vivo.
Databáze: OpenAIRE
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