Transgenic rice plants harboring the grain hardness-locus region of Aegilops tauschii
| Autor: | Sadequr Rahman, Hideo Wada, Go Suzuki, Yasuhiko Mukai, Takuya Deno, Haruna Oba, Hiromi Goto, Akiko Nakano |
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| Rok vydání: | 2011 |
| Předmět: |
DNA
Bacterial DNA Plant Transgene Genetic Vectors Agrobacterium Locus (genetics) Plant Science Biology Genes Plant Endosperm Transformation Genetic Botany Aegilops tauschii Transgenes Promoter Regions Genetic Gene Triticum Plant Proteins Genetics Oryza sativa food and beverages Oryza Promoter General Medicine Plants Genetically Modified biology.organism_classification Genetically modified rice Seeds Agronomy and Crop Science |
| Zdroj: | Plant Cell Reports. 30:2293-2301 |
| ISSN: | 1432-203X 0721-7714 |
| DOI: | 10.1007/s00299-011-1134-7 |
| Popis: | Grain hardness of wheat is determined by the hardness (Ha)-locus region, which contains three friabilin-related genes: puroindoline-a (Pina), puroindoline-b (Pinb) and GSP-1. In our previous study, we produced the transgenic rice plants harboring the large genomic fragment of the Ha-locus region of Aegilops tauschii containing Pina and GSP-1 genes by Agrobacterium-mediated transformation. To examine the effects of the transgenes in the rice endosperms, we firstly confirmed the homozygosity of the T-DNAs in four independent T2 lines by using fluorescence in situ hybridization (FISH) and DNA gel blot analyses. The transgenes, Pina and GSP-1, were stably expressed in endosperms of the T3 and T4 seeds at RNA and protein levels, indicating that the promoters and other regulatory elements on the wheat Ha-locus region function in rice, and that multigene transformation using a large genomic fragment is a useful strategy. The functional contribution of the transgene-derived friabilins to the rice endosperm structure was considered as an increase of spaces between compound starch granules, resulting in a high proportion of white turbidity seeds. |
| Databáze: | OpenAIRE |
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