Drosophila Tubulin-Specific Chaperone E Recruits Tubulin around Chromatin to Promote Mitotic Spindle Assembly
| Autor: | Romain Gibeaux, Jean-Philippe Gagné, Laurent Richard-Parpaillon, Emmanuel Gallaud, Régis Giet, Mathieu Métivier, Christelle Benaud, Guy G. Poirier, Alexandre Thomas, Denis Chrétien, Aude Pascal |
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| Přispěvatelé: | Institut de Génétique et Développement de Rennes (IGDR), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), CHU de Québec–Université Laval, Université Laval [Québec] (ULaval), Centre des Sciences du Goût (CSG), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS), Ligue Contre le Cancer, Université de Rennes 1, Centre National de la Recherche Scientifique, CDA00019/2019-C, Fondation pour la Recherche Médicale, Fondation ARC pour la Recherche contre le Cancer, Human Frontier Science Program, Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ) |
| Rok vydání: | 2020 |
| Předmět: |
CAP-Gly motif
mitotic spindle assembly 0301 basic medicine tubulin dynamics microtubule nucleation Mitosis [SDV.CAN]Life Sciences [q-bio]/Cancer [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC] Spindle Apparatus Microtubules General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine Tubulin Animals tubulin-specific chaperone E [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM] ComputingMilieux_MISCELLANEOUS neural stem cells Microtubule nucleation biology nuclear pore proteins Chromatin [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biomolecules [q-bio.BM] Cell biology Spindle apparatus [SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics Ran [SDV.BDD.EO]Life Sciences [q-bio]/Development Biology/Embryology and Organogenesis 030104 developmental biology Centrosome biology.protein Drosophila General Agricultural and Biological Sciences 030217 neurology & neurosurgery Lamin |
| Zdroj: | Current Biology-CB Current Biology-CB, Elsevier, 2020, ⟨10.1016/j.cub.2020.11.009⟩ Current Biology-CB, Elsevier, 2021, 31 (4), pp.684-695.e6. ⟨10.1016/j.cub.2020.11.009⟩ Current Biology-CB, 2021, 31 (4), pp.684-695.e6. ⟨10.1016/j.cub.2020.11.009⟩ |
| ISSN: | 1879-0445 0960-9822 |
| DOI: | 10.1016/j.cub.2020.11.009⟩ |
| Popis: | International audience; Proper assembly of mitotic spindles requires microtubule nucleation not only at the centrosomes but also around chromatin. In this study, we found that the Drosophila tubulin-specific chaperone dTBCE is required for the enrichment of tubulin in the nuclear space after nuclear envelope breakdown and for subsequent promotion of spindle microtubule nucleation. These events depend on the CAP-Gly motif found in dTBCE and are regulated by Ran and lamin proteins. Our data suggest that during early mitosis, dTBCE and nuclear pore proteins become enriched in the nucleus, where they interact with the Ran GTPase to promote dynamic tubulin enrichment. We propose that this novel mechanism enhances microtubule nucleation around chromatin, thereby facilitating mitotic spindle assembly. |
| Databáze: | OpenAIRE |
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