Ribosomal small subunit domains radiate from a central core
| Autor: | Loren Dean Williams, Burak Gulen, Nicholas V. Hud, Eric B. O’Neill, C. Denise Okafor, Anton S. Petrov, Drew Vander Wood |
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| Rok vydání: | 2016 |
| Předmět: |
Models
Molecular Ribosomal Proteins 0301 basic medicine Architecture domain Protein domain Ribosome Subunits Small Bacterial Immunoglobulin domain Computational biology Biology Article Domain (software engineering) 03 medical and health sciences RNA Transfer EVH1 domain Ribosomal protein RNA Ribosomal 16S B3 domain Base Pairing Genetics Multidisciplinary Base Sequence 030102 biochemistry & molecular biology Thermus thermophilus 030104 developmental biology Cyclic nucleotide-binding domain Mutation Nucleic Acid Conformation |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep20885 |
| Popis: | The domain architecture of a large RNA can help explain and/or predict folding, function, biogenesis and evolution. We offer a formal and general definition of an RNA domain and use that definition to experimentally characterize the rRNA of the ribosomal small subunit. Here the rRNA comprising a domain is compact, with a self-contained system of molecular interactions. A given rRNA helix or stem-loop must be allocated uniquely to a single domain. Local changes such as mutations can give domain-wide effects. Helices within a domain have interdependent orientations, stabilities and interactions. With these criteria we identify a core domain (domain A) of small subunit rRNA. Domain A acts as a hub, linking the four peripheral domains and imposing orientational and positional restraints on the other domains. Experimental characterization of isolated domain A, and mutations and truncations of it, by methods including selective 2′OH acylation analyzed by primer extension and circular dichroism spectroscopy are consistent with our architectural model. The results support the utility of the concept of an RNA domain. Domain A, which exhibits structural similarity to tRNA, appears to be an essential core of the small ribosomal subunit. |
| Databáze: | OpenAIRE |
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