Pathoadaptive Mutations in Salmonella enterica Isolated after Serial Passage in Mice
| Autor: | Henry S. Gibbons, Gary Ouellette, Carolyn Nicole Rosenzweig, Naeem Anwar, Ed Fochler, Dan I. Andersson, Sanna Koskiniemi, S. M. Broomall, Mark Karavis, Paul McGregor, Mikael Rhen, Alvin T. Liem, Linus Sandegren, Lauren A. McNew, Evan W. Skowronski |
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| Rok vydání: | 2013 |
| Předmět: |
Salmonella typhimurium
Bacterial Diseases Medicin och hälsovetenskap Salmonella Mouse Mutant lcsh:Medicine medicine.disease_cause Medical and Health Sciences Mice Serial passage Genes Regulator Genome Sequencing Serial Passage lcsh:Science Genetics Mice Inbred BALB C Mutation Multidisciplinary biology Genomics Animal Models Adaptation Physiological Bacterial Pathogens DNA-Binding Proteins Cell Motility Infectious Diseases Flagella Salmonella enterica DNA Nucleotidyltransferases Medicine Female Research Article Biophysics Flagellum Microbiology Model Organisms Bacterial Proteins medicine Animals Biology Gene Salmonella Infections Animal Bacterial Evolution Point mutation lcsh:R Computational Biology Bacteriology Gene Expression Regulation Bacterial Sequence Analysis DNA Flagellar Motility biology.organism_classification Microbial Evolution bacteria lcsh:Q Genome Bacterial Molecular Chaperones |
| Zdroj: | PLoS ONE, Vol 8, Iss 7, p e70147 (2013) PLoS ONE |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0070147 |
| Popis: | How pathogenic bacteria adapt and evolve in the complex and variable environment of the host remains a largely unresolved question. Here we have used whole genome sequencing of Salmonella enterica serovar Typhimurium LT2 populations serially passaged in mice to identify mutations that adapt bacteria to systemic growth in mice. We found unique pathoadaptive mutations in two global regulators, phoQ and stpA, which increase the competitive indexes of the bacteria 3- to 5-fold. Also, all mouse-adapted lineages had changed the orientation of the hin invertable element, resulting in production of a FliC type of flagellum. Competition experiments in mice with locked flagellum mutants showed that strains expressing the FliC type of flagellum had a 5-fold increase in competitive index as compared to those expressing FljB type flagellum. Combination of the flagellum cassette inversion with the stpA mutation increased competitive indexes up to 20-fold. These experiments show that Salmonella can rapidly adapt to a mouse environment by acquiring a few mutations of moderate individual effect that when combined confer substantial increases in growth. |
| Databáze: | OpenAIRE |
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