Introduction and expression of genes for metabolic engineering applications in Saccharomyces cerevisiae
| Autor: | Sneha Srikrishnan, Nancy A. Da Silva |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Genetics
Regulation of gene expression biology Saccharomyces cerevisiae Fungal genetics Gene Dosage General Medicine Computational biology biology.organism_classification Applied Microbiology and Biotechnology Microbiology Gene dosage Yeast Gene Expression Regulation Enzymologic Metabolic engineering Metabolic pathway Metabolic Engineering Gene Expression Regulation Fungal Biomass Promoter Regions Genetic Gene Metabolic Networks and Pathways |
| Zdroj: | FEMS yeast research. 12(2) |
| ISSN: | 1567-1364 |
| Popis: | Metabolic pathway engineering in the yeast Saccharomyces cerevisiae leads to improved production of a wide range of compounds, ranging from ethanol (from biomass) to natural products such as sesquiterpenes. The introduction of multienzyme pathways requires precise control over the level and timing of expression of the associated genes. Gene number and promoter strength/regulation are two critical control points, and multiple studies have focused on modulating these in yeast. This MiniReview focuses on methods for introducing genes and controlling their copy number and on the many promoters (both constitutive and inducible) that have been successfully employed. The advantages and disadvantages of the methods will be presented, and applications to pathway engineering will be highlighted. |
| Databáze: | OpenAIRE |
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