Effects of Mechanical Force on Primary Human Fibroblasts Derived from the Gingiva and the Periodontal Ligament
| Autor: | C. Theilig, Gabriele Leyhausen, August Bernd, R. Kaufmann, W. Geurtsen |
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| Rok vydání: | 2001 |
| Předmět: |
0301 basic medicine
Periodontal Ligament Gingiva Tenascin Cell Communication Statistics Nonparametric Extracellular matrix 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Lactate dehydrogenase Cell Adhesion Humans Periodontal fiber Centrifugation Cell adhesion General Dentistry Cells Cultured L-Lactate Dehydrogenase biology Cell growth Chemistry 030206 dentistry Adhesion Fibroblasts Extracellular Matrix Cell biology 030104 developmental biology Immunology biology.protein Stress Mechanical Cell Division Gravitation |
| Zdroj: | Journal of Dental Research. 80:1777-1780 |
| ISSN: | 1544-0591 0022-0345 |
| DOI: | 10.1177/00220345010800081901 |
| Popis: | Previous experiments have shown that mechanical stress may alter the interactions between cells and extracellular matrix (ECM). The purpose of our study was to investigate the effects of mechanical load on metabolism and ECM expression of primary human periodontal cells. The influence of gravitational force on proliferation, lactate dehydrogenase (LDH) release, and tenascin expression of gingival (HGF) and periodontal ligament fibroblasts (HPDL), as well as their adhesion to various extracellular matrix (ECM) components, was determined. Cells were centrifuged in microplates or flat tubes for 16 hrs at 217 g. Neither an enhanced release of LDH nor an alteration of cell proliferation could be detected after centrifugation. However, the attachment of loaded gingival and periodontal ligament fibroblasts to all tested ECM components significantly decreased in comparison with controls (Wilcoxon-Mann-Whitney test; HGF, p < 0.05; HPDL, p < 0.01). Tenascin expression of mechanically stressed fibroblasts significantly increased in comparison with controls (p < 0.01). |
| Databáze: | OpenAIRE |
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