Isolation of Kupffer Cells and Hepatocytes from a Single Mouse Liver
| Autor: | Cecilia Morgantini, Marcela Aparicio-Vergara, Myriam Aouadi, Michaela Tencerova, Emelie Barreby |
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| Přispěvatelé: | Borel, Florie, Mueller, Christian |
| Rok vydání: | 2017 |
| Předmět: | |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493971619 Aparicio-Vergara, M, Tencerova, M, Morgantini, C, Barreby, E & Aouadi, M 2017, Isolation of Kupffer Cells and Hepatocytes from a Single Mouse Liver . in F Borel & C Mueller (eds), Alpha-1 Antitrypsin Deficiency : Methods and Protocols . vol. 1639, Humana Press, Methods in Molecular Biology, vol. 1639, pp. 161-171 . https://doi.org/10.1007/978-1-4939-7163-3_16 |
| DOI: | 10.1007/978-1-4939-7163-3_16 |
| Popis: | Liver perfusion is a common technique used to isolate parenchymal and non-parenchymal liver cells for in vitro experiments. This method allows hepatic cells to be separated based on their size and weight, by centrifugation using a density gradient. To date, other methods allow the isolation of only one viable hepatic cellular fraction from a single mouse; either parenchymal (hepatocytes) or non-parenchymal cells (i.e., Kupffer cells or hepatic stellate cells). Here, we describe a method to isolate both hepatocytes and Kupffer cells from a single mouse liver, thereby providing the unique advantage of studying different liver cell types that have been isolated from the same organism. |
| Databáze: | OpenAIRE |
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