Biological effects of an oxyphytosterol generated by β-Sitosterol ozonization
| Autor: | Sayuri Miyamoto, Gláucia Maria Machado-Santelli, Bianca S. Takayasu, Anali M. B. Garnique, Janice Onuki, Miriam Uemi, Igor R. Martins |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Cell Survival Lysine Biophysics medicine.disease_cause Biochemistry Microtubules 03 medical and health sciences Ozone medicine Mitotic Index Humans MTT assay Viability assay Molecular Biology chemistry.chemical_classification Cell Nucleus Reactive oxygen species 030102 biochemistry & molecular biology Phytosterol Hep G2 Cells Cell cycle G1 Phase Cell Cycle Checkpoints Sitosterols Acetylcysteine Oxidative Stress 030104 developmental biology Mechanism of action chemistry medicine.symptom Oxidative stress |
| Zdroj: | Archives of biochemistry and biophysics. 696 |
| ISSN: | 1096-0384 |
| Popis: | β-Sitosterol (βSito) is the most abundant phytosterol found in vegetable oils, grains such as wheat, beans, and corn, and in many phytosterol-enriched foods. It is prone to oxidation by reactive oxygen species, such as ozone, leading to the formation of oxyphytosterols. A better understanding regarding the biological effects and mechanism of action of oxyphytosterols is required since the beneficial and adverse side effects of these compounds on human health remain highly controversial. In this work, we investigated the biological effects of β-Secosterol (βSec), a new oxyphytosterol generated by the reaction of βSito with ozone. Treatment of HepG2 cells with βSito or βSec (0.1–100 μM) for 24, 48, and 72 h induced a dose-dependent reduction of cell viability in the MTT assay, with βSec showing higher efficacy than βSito. However, βSec presented a lower potency than βSito, showing IC50 = 37.32 μM, higher than βSito (IC50 = 0.23 μM) at 48 h. Cell cycle analyses by flow cytometry showed a slight decrease of G0/G1 phase with βSito 0.5 μM, but a significant cell cycle arrest at the G0/G1 phase in the treatment for 48 h with βSec 20 μM (62.69 ± 2.15%, p l -cysteine was not able to inhibit any of these cellular effects, indicating a lack of involvement of oxidative stress in the mechanism of action of βSec. Although not further investigated in this study, it was discussed the hypothesis that covalent adduct formation with lysine residues of proteins, could play an important role in the biological effects elicited by βSec. Elucidation of the primary cellular processes induced by βSec provides the essential knowledge to be aware of its potential adverse side effects or therapeutic use of this oxyphytosterol. |
| Databáze: | OpenAIRE |
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