Endothelial cell activation following moderate traumatic brain injury
| Autor: | Harold Goldman, Cheri R. Owen, Paula Dore-Duffy, José A. Rafols, Roumen Balabanov, Gregory Pellizon, Sharon Murphy |
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| Rok vydání: | 2001 |
| Předmět: |
Male
Endothelium Amino Acid Transport System X-AG Vascular Cell Adhesion Molecule-1 Endothelial activation MHC class I E-selectin Receptors Transferrin medicine Image Processing Computer-Assisted Animals Rats Wistar MHC class II ICAM-1 biology Cell adhesion molecule Histocompatibility Antigens Class I Histocompatibility Antigens Class II General Medicine Intercellular Adhesion Molecule-1 Cell biology Rats Endothelial stem cell medicine.anatomical_structure Neurology Brain Injuries Cerebrovascular Circulation biology.protein ATP-Binding Cassette Transporters Neurology (clinical) Endothelium Vascular E-Selectin Biomarkers |
| Zdroj: | Neurological research. 23(2-3) |
| ISSN: | 0161-6412 |
| Popis: | Traumatic brain injury (TBI) initiates a cascade of acute and chronic injury responses which include disturbances in the cerebrovasculature that may result in the activation of the microvascular endothelial development of a dysfunction endothelium. The present study examines endothelial cell (EC) activation in a percussion model of moderate TBI. The criteria for endothelial activation used in these studies was surface expression of a number of markers collectively termed endothelial activation antigens. Temporal induction of the major histocompatibility (MHC) class II molecules, E-selectin (CD62E), vascular cell adhesion molecule (VACM-1) (CD106) as well as altered expression of constitutively expressed intercellular adhesion molecule-1 (ICAM-1) (CD54), the glucose transporter protein (glut-1), the transferrin receptor (tfR) (CD71), and MHC class I molecules was examined at various times following impact. Induction of E-selectin and increased expression of ICAM-1 was seen by 2 h post-impact (PI) and was sustained through 24 h PI. Decreased expression of immunologically reactive glut-1 and tfR was observed by 2-4 h PI and remained low up to 24 h PI. No induction of VCAM-1, MHC class II molecules or altered constitutive expression or MHC class I molecules was seen. Changes in EC activation were observed predominantly at the site of impact and were diminished temporarily. These results indicate that mild concussive injury to the brain results in activation of the endothelium. |
| Databáze: | OpenAIRE |
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