Efficacy of origanum syriacum essential oil against the mosquito vector culex quinquefasciatus and the gastrointestinal parasite anisakis simplex, with insights on acetylcholinesterase inhibition
| Autor: | Riccardo Petrelli, Domenico Otranto, Loredana Cappellacci, Francisco Les, Roman Pavela, Filippo Maggi, Angelo Canale, Veronica Galiffa, Stefano Dall'Acqua, Giovanni Benelli, Víctor López, Fabrizio Bartolucci, Carlota Gómez-Rincón, Stefania Sut |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
anisakiasis
contact toxicity enzyme inhibition fumigation toxicity larvicide mosquito control penetration assay Animals Anisakis Cholinesterase Inhibitors Culex Cymenes Larva Mosquito Vectors Oils Volatile Origanum Plant Leaves Plant Oils Anisakiasis Contact toxicity Enzyme inhibition Fumigation toxicity Larvicide Mosquito control Penetration assay Volatile Pharmaceutical Science Analytical Chemistry law.invention chemistry.chemical_compound 0302 clinical medicine law Drug Discovery Carvacrol 0303 health sciences biology Traditional medicine Chemistry (miscellaneous) Origanum syriacum Molecular Medicine 030231 tropical medicine Article lcsh:QD241-441 03 medical and health sciences lcsh:Organic chemistry parasitic diseases Toxicología Physical and Theoretical Chemistry Essential oil 030304 developmental biology Organic Chemistry Anisakis simplex fungi biology.organism_classification Antiparasitic agent Culex quinquefasciatus chemistry Oils |
| Zdroj: | R-USJ: Repositorio Institucional de la Universidad San Jorge Universidad San Jorge (USJ) Molecules Volume 24 Issue 14 Molecules, Vol 24, Iss 14, p 2563 (2019) |
| Popis: | Developing effective and eco-friendly antiparasitic drugs and insecticides is an issue of high importance nowadays. In this study, we evaluated the anthelminthic and insecticidal potential of the leaf essential oil obtained from Origanum syriacum against the L3 larvae of the parasitic nematode Anisakis simplex and larvae and adults of the mosquito Culex quinquefasciatus. Tests on A. simplex were performed by standard larvicidal and penetration assays, while mosquito toxicity was assessed relying on larvicidal, tarsal contact, and fumigation tests. To shed light on the possible mode of action, we analyzed the oil impact as acetylcholinesterase (AChE) inhibitor. This oil was particularly active on L3 larvae of A. simplex, showing a LC50 of 0.087 and 0.067 mg mL&minus 1 after 24 and 48 h treatment, respectively. O. syriacum essential oil was highly effective on both larvae and adults of C. quinquefasciatus, showing LC50 values of 32.4 mg L&minus 1 and 28.1 µ g cm&minus 2, respectively. Its main constituent, carvacrol, achieved larvicidal LC50(90) of 29.5 and 39.2 mg L&minus 1, while contact toxicity assays on adults had an LC50(90) of 25.5 and 35.8 µ 2, respectively. In fumigation assays, the LC50 was 12.1 µ L L&minus 1 after 1 h and decreased to 1.3 µ 1 in 24 h of exposure. Similarly, the fumigation LC50 of carvacrol was 8.2 µ 1 after 1 h of exposure, strongly decreasing to 0.8 µ 1 after 24 h of exposure. These results support the folk usage of Lebanese oregano as an antiparasitic agent, providing new insights about its utilization for developing new effective and eco-friendly nematocidal and insecticidal products. |
| Databáze: | OpenAIRE |
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