Validation of ELISA Kits for Pesticide Residue Analysis in Vegetables and Fruits
| Autor: | Isao Saito, Tomomi Yamasaki, Yuki Hirakawa, Shiro Miyake, Tomomi Inoue, Eiji Ueno |
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| Rok vydání: | 2015 |
| Předmět: |
Crops
Agricultural Pesticide residue Chlorothalonil business.industry Pesticide Residues Reproducibility of Results Enzyme-Linked Immunosorbent Assay General Medicine Repeatability Pesticide Acetamiprid Biotechnology Fenitrothion Matrix (chemical analysis) chemistry.chemical_compound chemistry Azoxystrobin Fruit Vegetables Reagent Kits Diagnostic Food science business |
| Zdroj: | Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi). 56:240-246 |
| ISSN: | 1882-1006 0015-6426 |
| Popis: | Five kinds of commercially available ELISA kits (acetamiprid, azoxystrobin, chlorothalonil, fenitrothion and imidacloprid) were validated for determination of pesticide residues in vegetables and fruits. The reaction characteristics were also examined to evaluate their influence on the determinations. The trueness value was 91-162%, the repeatability was 2.1-16.2%, and the reproducibility was 4.0-20.3%. The desired values were achieved for 18 among 30 combinations (60%) of the ELISA kits and the agricultural products examined. A standard curve was necessary for each of the ELISA examinations. The matrix of the agricultural products and pipetting skill of the lab technician both influenced the measurment results. |
| Databáze: | OpenAIRE |
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