Molecular Characterization of Cyst Nematode Species (Heterodera spp.) from the Mediterranean Basin using RFLPs and Sequences of ITS-rDNA
| Autor: | Nicola Vovlas, Mehrdad Madani, Maurice Moens, Pablo Castillo, Sergei A. Subbotin |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
Heterodera humuli
Physiology Heterodera ripae Plant Science Heterodera hordecalis Heterodera ciceri Heterodera schachtii Botany Heterodera mediterranea Genetics Heterodera fici Heterodera filipjevi biology Heterodera biology.organism_classification Heterodera carotae Internal transcribed spacer region Molecular identification Restriction fragment length polymorphism Agronomy and Crop Science Phylogenetic relationships Heterodera goettingiana |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | full text: http://www.ias.csic.es/pcastillo/journal%20of%20phytopathology_152_229-234.pdf Fifteen populations of cyst-forming nematodes belonging to 11 known and one unidentified species collected in countries bordering the Mediterranean Sea were studied using polymerase chain reaction restriction fragment length polymorphism (PCR–RFLP) and internal transcribed spacer (ITS)-rDNA sequences. RFLP profiles generated by the restriction enzymes AluI, AvaI, Bsh1236I, HaeIII, Hin6I, MvaI, PstI and RsaI are presented for Heterodera carotae, H. ciceri, H. fici, H. filipjevi, H. goettingiana, H. hordecalis, H. humuli, H. mediterranea, H. ripae and H. schachtii. Molecular data support the first detection of H. filipjevi from wheat in Italy and H. ripae from nettle in Greece. A relative high level of sequence divergence between populations of H. hordecalis was observed. This suggests that two species might presently be grouped under this taxon. The phylogenetic relationship between the Mediterranean cyst-forming nematode species is analysed based on the ITS-rDNA sequences. |
| Databáze: | OpenAIRE |
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