Cocaine and Caffeine Effects on the Conditioned Place Preference Test: Concomitant Changes on Early Genes within the Mouse Prefrontal Cortex and Nucleus Accumbens
| Autor: | Javier A. Muñiz, José P. Prieto, Betina González, Máximo H. Sosa, Jean L. Cadet, Cecilia Scorza, Francisco J. Urbano, Verónica Bisagno |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
CAFFEINE CIENCIAS MÉDICAS Y DE LA SALUD nucleus accumbens Cognitive Neuroscience medicine.medical_treatment Inmunología cocaine Context (language use) Pharmacology Nucleus accumbens immediate-early genes LEARNING lcsh:RC321-571 03 medical and health sciences Behavioral Neuroscience chemistry.chemical_compound 0302 clinical medicine Dopamine medicine Prefrontal cortex lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry Original Research caffeine prefrontal cortex learning IMMEDIATE-EARLY GENES purl.org/becyt/ford/3.1 [https] Conditioned place preference Stimulant Medicina Básica 030104 developmental biology Neuropsychology and Physiological Psychology chemistry purl.org/becyt/ford/3 [https] Psychology Caffeine COCAINE Neuroscience 030217 neurology & neurosurgery medicine.drug FOSB |
| Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET Frontiers in Behavioral Neuroscience Frontiers in Behavioral Neuroscience, Vol 11 (2017) |
| DOI: | 10.3389/fnbeh.2017.00200/full |
| Popis: | Caffeine is the world's most popular psychostimulant and is frequently used as an active adulterant in many illicit drugs including cocaine. Previous studies have shown that caffeine can potentiate the stimulant effects of cocaine and cocaine-induced drug seeking behavior. However, little is known about the effects of this drug combination on reward-related learning, a key process in the maintenance of addiction and vulnerability to relapse. The goal of the present study was thus to determine caffeine and cocaine combined effects on the Conditioned Place Preference (CPP) test and to determine potential differential mRNA expression in the Nucleus Accumbens (NAc) and medial prefrontal cortex (mPFC) of immediate-early genes (IEGs) as well as dopamine and adenosine receptor subunits. Mice were treated with caffeine (5 mg/kg, CAF), cocaine (10 mg/kg, COC), or their combination (caffeine 5 mg/kg + cocaine 10 mg/kg, CAF-COC) and trained in the CPP test or treated with repeated injections inside the home cage. NAc and mPFC tissues were dissected immediately after the CPP test, after a single conditioning session or following psychostimulant injection in the home cage for mRNA expression analysis. CAF-COC induced a marked change of preference to the drug conditioned side of the CPP and a significant increase in locomotion compared to COC. Gene expression analysis after CPP test revealed specific up-regulation in the CAF-COC group of Drd1a, cFos, and FosB in the NAc, and cFos, Egr1, and Npas4 in the mPFC. Importantly, none of these changes were observed when animals received same treatments in their home cage. With a single conditioning session, we found similar effects in both CAF and CAF-COC groups: increased Drd1a and decreased cFos in the NAc, and increased expression of Drd1a and Drd2, in the mPFC. Interestingly, we found that cFos and Npas4 gene expression were increased only in the mPFC of the CAF-COC. Our study provides evidence that caffeine acting as an adulterant could potentiate reward-associated memories elicited by cocaine. This is associated with specific changes in IEGs expression that were observed almost exclusively in mice that received the combination of both psychostimulants in the context of CPP memory encoding and retrieval. Our results highlight the potential relevance of caffeine in the maintenance of cocaine addiction which might be mediated by modifying neural plasticity mechanisms that strengthen learning of the association between drug and environment. Fil: Muñiz, Javier Andrés. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Prieto, Julián José. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay Fil: Gonzalez, Betina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Sosa, Máximo Hernán. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cadet, Jean L.. National Institute on Drug Abuse; Estados Unidos Fil: Scorza, Cecilia. Instituto de Investigaciones Biológicas "Clemente Estable"; Uruguay Fil: Urbano Suarez, Francisco Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina Fil: Bisagno, Veronica. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
| Databáze: | OpenAIRE |
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