Natural Human Interferon-γ Derived from Lipopolysaccharide-stimulated Human Myelomonocytic HBL-38 Cells
| Autor: | Tadao Tanimoto, Masashi Kurimoto, Osamu Sano, Tsunetaka Ohta, Osamu Andoh, Hiroshi Yamauchi, Shunsaku Ando, Kakuji Torigoe |
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| Rok vydání: | 1988 |
| Předmět: |
Lipopolysaccharides
Cancer Research Lipopolysaccharide Cell division LPS‐stimulation Molecular Sequence Data Biology Monocytes Article Cell Line Natural human IFN‐γ Interferon-gamma chemistry.chemical_compound Antigen In vivo Interferon Cricetinae medicine Animals Humans Interferon gamma Amino Acid Sequence Interferon inducer Mass production Virology Molecular biology Myelomonocytic cell line Oncology chemistry Cell culture Antigens Surface Cell Division medicine.drug |
| Zdroj: | Japanese Journal of Cancer Research : Gann |
| ISSN: | 0910-5050 |
| DOI: | 10.1111/j.1349-7006.1988.tb02233.x |
| Popis: | A human myelomonocytic cell line, HBL-38 cells, propagated in vivo, spontaneously produced interferon (IFN)-gamma and IFN-alpha. Whereas hemmagglutinating virus of Japan (HVJ) enhanced the production of IFN-alpha, bacterial lipopolysaccharide (LPS) markedly enhanced the production of IFN-gamma. LPS could be replaced with lipid A. Furthermore, the enhancement of production of IFN-gamma by LPS was completely abolished by polymixin B. IFN-gamma derived from LPS-stimulated HBL-38 cells was purified to homogeneity and characterized. The apparent molecular weight, subspecies composition, amino acid sequence and glycosylated sites were in agreement with those of the product of normal human peripheral blood lymphocytes (PBL). These results indicate that the myelomonocytic HBL-38 cells, not a T-cell line, can also produce IFN-gamma identical to the product of normal human PBL. |
| Databáze: | OpenAIRE |
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