Observing the cell in its native state: Imaging subcellular dynamics in multicellular organisms
| Autor: | Abraham Q. Kohrman, Kishore R. Mosaliganti, Kai Wang, Srigokul Upadhyayula, Hanako Yashiro, David Q. Matus, Elliot M. Meyerowitz, Yuan Ruan, Tom W. Hiscock, Brian Cunniff, Dirk Hockemeyer, David G. Drubin, Minoru Koyama, Eric Betzig, Zach M. Collins, Ryan Forster, Ved P. Singh, Daniel E. Milkie, Ian A. Swinburne, Daphné Dambournet, Benjamin L. Martin, Tsung-Li Liu, Sean G. Megason, Steffen Scholpp, Tom Kirchhausen, Jamien Shea, Taylor N Medwig |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
General Science & Technology 1.1 Normal biological development and functioning Cell Mitosis Bioengineering Lattice light-sheet microscopy Endocytosis Eye Article Imaging 03 medical and health sciences 0302 clinical medicine Imaging Three-Dimensional Single-cell analysis Underpinning research Cell Movement Organelle Genetics medicine Animals Humans Zebrafish Organelles Microscopy Multidisciplinary biology biology.organism_classification Phenotype Cell biology Multicellular organism 030104 developmental biology medicine.anatomical_structure Three-Dimensional Cancer cell Biomedical Imaging Generic health relevance Single-Cell Analysis 030217 neurology & neurosurgery |
| Zdroj: | Science (New York, N.Y.), vol 360, iss 6386 |
| Popis: | True physiological imaging of subcellular dynamics requires studying cells within their parent organisms, where all the environmental cues that drive gene expression, and hence the phenotypes we actually observe, are present. A complete understanding also requires volumetric imaging of the cell and its surroundings at high spatiotemporal resolution without inducing undue stress on either. We combined lattice light sheet microscopy with two-channel adaptive optics to achieve, across large multicellular volumes, noninvasive aberration-free imaging of subcellular processes, including endocytosis, organelle remodeling during mitosis, and the migration of axons, immune cells, and metastatic cancer cells in vivo. The technology reveals the phenotypic diversity within cells across different organisms and developmental stages, and may offer insights into how cells harness their intrinsic variability to adapt to different physiological environments.One Sentence SummaryCombining lattice light sheet microscopy with adaptive optics enables high speed, high resolution in vivo 3D imaging of dynamic processes inside cells under physiological conditions within their parent organisms. |
| Databáze: | OpenAIRE |
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