Actomyosin tension is required for correct recruitment of adherens junction components and zonula occludens formation
| Autor: | Koji Nishimura, Hiroshi Hosoya, Naoko Inoue, Shigenobu Yonemura, Nagatoki Kinoshita, Yuka Miyake |
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| Rok vydání: | 2005 |
| Předmět: |
rho GTP-Binding Proteins
Pyridines macromolecular substances Biology Occludin Heterocyclic Compounds 4 or More Rings Cell Line Tight Junctions Adherens junction Mice Dogs Microscopy Electron Transmission Myosin Chlorocebus aethiops Animals Enzyme Inhibitors RNA Small Interfering Actin Myosin Type II Platelet-Derived Growth Factor Nonmuscle Myosin Type IIB Tight junction Cadherin Membrane Proteins Cell Biology Actomyosin Adherens Junctions Vinculin Amides Cell biology Rats Cytoskeletal Proteins COS Cells biology.protein Catenin complex Lysophospholipids Cell Adhesion Molecules |
| Zdroj: | Experimental cell research. 312(9) |
| ISSN: | 0014-4827 |
| Popis: | The adherens junction (AJ) densely associated with actin filaments is a major cell-cell adhesion structure. To understand the importance of actin filament association in AJ formation, we first analyzed punctate AJs in NRK fibroblasts where one actin cable binds to one AJ structure unit. The accumulation of AJ components such as the cadherin/catenin complex and vinculin, as well as the formation of AJ-associated actin cables depended on Rho activity. Inhibitors for the Rho target, ROCK, which regulates myosin II activity, and for myosin II ATPase prevented the accumulation of AJ components, indicating that myosin II activity is more directly involved than Rho activity. Depletion of myosin II by RNAi showed similar results. The inhibition of myosin II activity in polarized epithelial MTD-1A cells affected the accumulation of vinculin to circumferential AJ (zonula adherens). Furthermore, correct zonula occludens (tight junction) formation along the apicobasal axis that requires cadherin activity was also impaired. Although MDCK cells which are often used as typical epithelial cells do not have a typical zonula adherens, punctate AJs formed dependently on myosin II activity by inducing wound closure in a MDCK cell sheet. These findings suggest that tension generated by actomyosin is essential for correct AJ assembly. |
| Databáze: | OpenAIRE |
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