Phosphorylation of CENP-A on serine 7 does not control centromere function
| Autor: | Glennis A. Logsdon, Aaron Aslanian, Don W. Cleveland, Ben E. Black, Daniele Fachinetti, Solène Hervé, Andrea Scelfo, Yael Nechemia-Arbely, Viviana Barra, Sebastian Hoffmann |
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| Přispěvatelé: | Barra V., Logsdon G.A., Scelfo A., Hoffmann S., Herve S., Aslanian A., Nechemia-Arbely Y., Cleveland D.W., Black B.E., Fachinetti D., Stabilité Génétique et Oncogenèse (UMR 8200), Université Paris-Sud - Paris 11 (UP11)-Institut Gustave Roussy (IGR)-Centre National de la Recherche Scientifique (CNRS), Dept Computer Science, Florida State University, Florida State University [Tallahassee] (FSU), Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, University of California, University of California [San Diego] (UC San Diego), University of California-University of California-Ludwig Institute for Cancer Research - Department of Cellular and Molecular Medicine |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
1.1 Normal biological development and functioning Science [SDV]Life Sciences [q-bio] Centromere General Physics and Astronomy 02 engineering and technology [SDV.BC]Life Sciences [q-bio]/Cellular Biology macromolecular substances Biology General Biochemistry Genetics and Molecular Biology Article Serine Chromosome segregation 03 medical and health sciences Histone H3 Underpinning research Genetics Humans Viability assay Phosphorylation lcsh:Science ComputingMilieux_MISCELLANEOUS Cancer Gene Editing Multidisciplinary Gene targeting General Chemistry 021001 nanoscience & nanotechnology Cell biology Settore BIO/18 - Genetica 030104 developmental biology Chromosome segragation Hela Cells Epigenetics lcsh:Q Generic health relevance 0210 nano-technology Function (biology) Centromere Protein A Human HeLa Cells |
| Zdroj: | Nature Communications Nature communications, vol 10, iss 1 Nature Communications, Vol 10, Iss 1, Pp 1-10 (2019) Nature Communications, Nature Publishing Group, 2019, 10 (1), ⟨10.1038/s41467-018-08073-1⟩ Nature Communicqtions |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-018-08073-1⟩ |
| Popis: | CENP-A is the histone H3 variant necessary to specify the location of all eukaryotic centromeres via its CENP-A targeting domain and either one of its terminal regions. In humans, several post-translational modifications occur on CENP-A, but their role in centromere function remains controversial. One of these modifications of CENP-A, phosphorylation on serine 7, has been proposed to control centromere assembly and function. Here, using gene targeting at both endogenous CENP-A alleles and gene replacement in human cells, we demonstrate that a CENP-A variant that cannot be phosphorylated at serine 7 maintains correct CENP-C recruitment, faithful chromosome segregation and long-term cell viability. Thus, we conclude that phosphorylation of CENP-A on serine 7 is dispensable to maintain correct centromere dynamics and function. Phosphorylation of CENP-A on serine 7 has been proposed to control centromere assembly and function. Here, the authors use gene targeting at both endogenous CENP-A alleles and gene replacement in human cells to demonstrate that CENP-A that cannot be phosphorylated at serine 7 maintains correct CENP-C recruitment, faithful chromosome segregation and long-term cell viability. |
| Databáze: | OpenAIRE |
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