Structural insights into the NAD+-dependent formate dehydrogenase mechanism revealed from the NADH complex and the formate NAD+ ternary complex of the Chaetomium thermophilum enzyme
| Autor: | Simone Antonio De Rose, Berin Yilmazer, Huri Bulut, Michail N. Isupov, Jens C. Benninghoff, Barış Binay, Jennifer A. Littlechild |
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| Přispěvatelé: | İstinye Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Bulut, Huri |
| Rok vydání: | 2020 |
| Předmět: |
chemistry.chemical_classification
0303 health sciences biology Stereochemistry 030302 biochemistry & molecular biology Substrate (chemistry) Cofactor Catalysis 03 medical and health sciences chemistry.chemical_compound X-ray Crystallography Chaetomium thermophilum chemistry Mutagenesis Oxidoreductase Structural Biology Catalytic Mechanism biology.protein Formate NAD+ kinase Ternary complex 030304 developmental biology |
| Zdroj: | Journal of Structural Biology. 212:107657 |
| ISSN: | 1047-8477 |
| Popis: | The removal of carbon dioxide from the waste streams of industrial processes is a major challenge for creation of a sustainable circular economy. This makes the synthesis of formate from CO2 by NAD+ dependent formate dehydrogenases (FDHs) an attractive process for this purpose. The efficiency of this reaction is however low and to achieve a viable industrial process an optimised engineered enzyme needs to be developed. In order to understand the detailed enzymatic mechanism of catalysis structures of different cofactor and substrate complexes of the FDH from the thermophilic filamentous fungus, Chaetomium thermophilum have been determined to 1.2–1.3 Å resolution. The substrate formate is shown to be held by four hydrogen bonds in the FDH catalytic site within the ternary complex with substrate and NAD+and a secondary formate binding site is observed in crystals soaked with substrate. Water molecules are excluded from the FDH catalytic site when the substrate is bound. The angle between the plane of the NAD+ cofactor pyridine ring and the plane of the formate molecule is around 27°. Additionally, structures of a FDH mutant enzyme, N120C, in complex with the reduced form of the cofactor have also been determined both in the presence and absence of formate bound at the secondary site. These structures provide further understanding of the catalytic mechanism of this fungal enzyme. WOS:000600997800021 33148525 Q3 |
| Databáze: | OpenAIRE |
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