A promising natural product, pristimerin, results in cytotoxicity against breast cancer stem cells in vitro and xenografts in vivo through apoptosis and an incomplete autopaghy in breast cancer

Autor: Ayça Üvez, Mattia Mori, Konstantinos Dimas, Cinzia Ingallina, Pınar Alper, Chrisiida Tsimplouli, Ilaria D'Acquarica, Nazlihan Aztopal, Merve Erkisa, Elif Ilkay Armutak, Bruno Botta, Bulent Ozpolat, Simone Berardozzi, Evangelia Sereti, Didem Karakas, Buse Cevatemre, Ebru Gürel Gürevin, Engin Ulukaya
Přispěvatelé: Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Bölümü., Cevatemre, Buse, Erkısa, Merve, Aztopal, Nazlıhan, Karakaş, Didem, Alper, Pınar, AHD-2050-2022, AAM-1001-2020, L-6687-2018, L-6682-2018, İstinye Üniversitesi, Rektörlük, Didem Karakaş / 0000-0002-3781-6834, Engin Ulukaya / 0000-0003-4875-5472, Merve Erkısa Genel / 0000-0002-3127-742X, Pınar Alper / 0000-0001-9631-3551, Erkisa Genel, Merve, Karakaş Zeybek, Didem, Ulukaya, Engin, Didem Karakaş / L-6682-2018, Engin Ulukaya / K-5792-2018, Merve Erkısa Genel / AAM-1001-2020, Pınar Alper / DUL-1586-2022, Didem Karakaş / 56422040600, Engin Ulukaya / 6602927353, Merve Erkısa Genel / 57126208900, Pınar Alper / 57197858774
Rok vydání: 2018
Předmět:
0301 basic medicine
Palladium(ıı)
Protein bcl 2
Unclassified drug
Mouse
Proliferation
Enzyme activation
Apoptosis
Antiproliferative activity
IC50
MCF-7 cell line
Experimental mammary neoplasm
Cell vacuole
Animal tissue
Metastasis
Western blotting
Biological product
Unfolded protein response
Degradation
Mice
In vivo study
Breast cancer
0302 clinical medicine
Antineoplastic agents
Cytotoxic T cell
Flow cytometry
Priority journal
Caspase 7
education.field_of_study
Signaling pathway
Caspase 3
Chemistry
Neoplastic stem cells
Antineoplastic agent
Protein cleavage
Drug screening
Sesquiterpene
Pristimerin
Maytenus
030220 oncology & carcinogenesis
Sequestosome 1
cytoplasmic vacuolation
endoplasmic reticulum stress
Female
triterpenoid
Stem cell
Pentacyclic Triterpenes
Animal cell
Drug mechanism
Xenograft model antitumor assays
Human
Drug cytotoxicity
Programmed cell death
Microtubule associated protein
Microtubule associated protein 1A 1B light chain 3B
mammosphere
Population
Article
03 medical and health sciences
Cancer stem cell
Autophagy
Animals
Humans
Animal model
Drug dose comparison
Biological products
Animal experiment
ATPase activity assay
education
Antineoplastic activity
Pharmacology
Pharmacology & pharmacy
Animal
Carcinoma
In vitro study
Tumor cell line
Nonhuman
Triterpenes
Mammary neoplasms
experimental
Drug effect
Drug efficacy
Cell line
tumor
030104 developmental biology
Human cell
Doxorubicin
Cancer cell
Cancer research
Protein expression
Tumor xenograft
Triterpene
Controlled study
Phosphatidylinositol 3 kinase
Zdroj: Pharmacological Research. 129:500-514
ISSN: 1043-6618
Popis: WOS: 000428102600047 Several natural products have been suggested as effective agents for the treatment of cancer. Given the important role of CSCs (Cancer Stem Cells) in cancer, which is a trendy hypothesis, it is worth investigating the effects of pristimerin on CSCs as well as on the other malignant cells (MCF-7 and MDA-MB-231) of breast cancer. The anti-growth activity of pristimerin against MCF-7 and MCF-7s (cancer stem cell enriched population) cells was investigated by real time viability monitorization (xCELLigence System (R)) and ATP assay, respectively. Mode of cell death was evaluated using electron and fluorescence microscopies, western blotting (autophagy, apoptosis and ER-stress related markers) and flow cytometry (annexin-V staining, caspase 3/7 activity, BCL-2 and PI3K expressions). Pristimerin showed an anti-growth effect on cancer cells and cancer stem cells with IC50 values ranging at 0.38-1.75 mu M. It inhibited sphere formation at relatively lower doses (
Databáze: OpenAIRE
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