Triangulating Nucleic Acid Conformations Using Multicolor Surface Energy Transfer
| Autor: | Ryan A. Riskowski, Geoffrey F. Strouse, Rachel E. Armstrong, Nancy L. Greenbaum |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification Range (particle radiation) biology Chemistry Biomolecule Energy transfer General Engineering Analytical chemistry Ribozyme General Physics and Astronomy Metal Nanoparticles DNA Fluorescence 03 medical and health sciences 030104 developmental biology Förster resonance energy transfer Nucleic Acid Conformations biology.protein Fluorescence Resonance Energy Transfer Nucleic Acid Conformation General Materials Science Surface energy transfer Biological system |
| Zdroj: | ACS nano. 10(2) |
| ISSN: | 1936-086X |
| Popis: | Optical ruler methods employing multiple fluorescent labels offer great potential for correlating distances among several sites, but are generally limited to interlabel distances under 10 nm and suffer from complications due to spectral overlap. Here we demonstrate a multicolor surface energy transfer (McSET) technique able to triangulate multiple points on a biopolymer, allowing for analysis of global structure in complex biomolecules. McSET couples the competitive energy transfer pathways of Förster Resonance Energy Transfer (FRET) with gold-nanoparticle mediated Surface Energy Transfer (SET) in order to correlate systematically labeled points on the structure at distances greater than 10 nm and with reduced spectral overlap. To demonstrate the McSET method, the structures of a linear B-DNA and a more complex folded RNA ribozyme were analyzed within the McSET mathematical framework. The improved multicolor optical ruler method takes advantage of the broad spectral range and distances achievable when using a gold nanoparticle as the lowest energy acceptor. The ability to report distance information simultaneously across multiple length scales, short-range (10-50 Å), mid-range (50-150 Å), and long-range (150-350 Å), distinguishes this approach from other multicolor energy transfer methods. |
| Databáze: | OpenAIRE |
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