Monoclonal antibody recognizing SLLTEVET epitope of M2 protein potently inhibited the replication of influenza A viruses in MDCK cells
| Autor: | Tianhou Wang, Licheng Zhou, Xu-Guang Xi, Hongwei Xu, Yongjin Wang, Xiaoming Wang, Huiling Shi, Tetsuya Toyoda, Hong Yao |
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| Přispěvatelé: | East China Normal University [Shangaï] (ECNU), Génotoxicologie et cycle cellulaire (GCC), Institut Curie [Paris]-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur de Shanghai, Académie des Sciences de Chine - Chinese Academy of Sciences (IPS-CAS), Réseau International des Instituts Pasteur (RIIP), This work was supported by grants from Shanghai Municipal Science and Technology Commission (No. 07DZ22940) and Shanghai Municipal Wildlife Administration (No. SBHZ2006_01)., We thank Dr. Yoshihiro Kawaoka from University of Madison-Wisconsin for providing the reverse genetics system for generating A/WSN/33 (H1N1) viruses. |
| Rok vydání: | 2009 |
| Předmět: |
MESH: Virus Replication
[SDV]Life Sciences [q-bio] Peptide binding Influenza A MESH: Amino Acid Sequence Virus Replication medicine.disease_cause Biochemistry Epitope MESH: Dogs Epitopes Influenza A virus MESH: Animals Inhibition chemistry.chemical_classification 0303 health sciences Antibodies Monoclonal 3. Good health Amino acid MESH: Viral Matrix Proteins/immunology Ectodomain Influenza Vaccines M2e8 MESH: Epitopes/genetics Antibody MESH: Viral Matrix Proteins/genetics Monoclonal antibody MESH: Mutation medicine.drug_class MESH: Influenza Vaccines/immunology Molecular Sequence Data Biophysics MESH: Antibodies Monoclonal/immunology Biology Cell Line Viral Matrix Proteins 03 medical and health sciences Dogs MESH: Epitopes/chemistry MESH: Viral Matrix Proteins/chemistry MESH: Influenza A virus/physiology medicine Animals Amino Acid Sequence Molecular Biology 030304 developmental biology MESH: Molecular Sequence Data MESH: Epitopes/immunology 030306 microbiology Cell Biology Virology Molecular biology In vitro MESH: Cell Line chemistry Mutation biology.protein |
| Zdroj: | Biochemical and Biophysical Research Communications Biochemical and Biophysical Research Communications, Elsevier, 2009, 385 (1), pp.118-122. ⟨10.1016/j.bbrc.2009.04.129⟩ |
| ISSN: | 0006-291X 1090-2104 |
| DOI: | 10.1016/j.bbrc.2009.04.129 |
| Popis: | International audience; The ectodomain of influenza A virus M2 protein (M2e) is composed of 24 amino acids and induces antibodies with inhibitory effect against a broad spectrum of influenza A subtypes in vitro and in vivo. Although relatively conserved, 21 M2e variants emerged in recent influenza A strains, most of the mutations appeared in the middle part of M2e domain. In this study, we characterized the in vitro inhibition efficacy of a monoclonal antibody (mAb) M2e8-7 recognizing the N terminus highly conserved epitope SLLTEVET (aa 2-9) which is common for both M1 and M2 proteins. Peptide binding assay showed that mAb M2e8-7 reacted strongly with M2e and 19 M2e variant peptides. The mAb M2e8-7 potently inhibited the replication of influenza A virus H1 and H3 subtypes in MDCK cells. Two important amino acids in M2e epitope, Threonine at position five and the Glutamic acid at position six, were identified to lead antibody-escaping variants. These results brought new insight in developing vaccine and therapeutic agents against influenza A virus infections. |
| Databáze: | OpenAIRE |
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