A new metal-free near-infrared fluorescent probe based on nitrofuran for the detection and bioimaging of carbon monoxide releasing molecule-2
Autor: | Xiaojie Tang, Chengxiao Zhang, Yuan Zhang, Yangxiong Li, Qiuyue Wang, Zhao Li |
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Rok vydání: | 2021 |
Předmět: |
Detection limit
Carbon monoxide-releasing molecule-2 Carbon Monoxide Chemistry medicine.drug_class Nitrofurans Near-infrared spectroscopy Biochemistry Combinatorial chemistry Fluorescence Analytical Chemistry chemistry.chemical_compound Mice In vivo Electrochemistry Nitro medicine Organometallic Compounds Environmental Chemistry Animals Nitrofuran Spectroscopy Zebrafish Carbon monoxide Fluorescent Dyes |
Zdroj: | The Analyst. 147(2) |
ISSN: | 1364-5528 |
Popis: | As a stable donor for releasing controlled amounts of CO, carbon monoxide releasing molecule-2 (CORM-2) is a new type of therapeutic drug that contributes to exploring the pathophysiological effects of CO. The accurate detection of CORM-2 in biological systems is of great significance for controlling its dosage as a therapeutic drug and elucidating the reaction mechanisms of CO, but currently there is a lack of metal-free near-infrared fluorescent probes. Herein, a new metal-free near-infrared fluorescent probe based on nitrofuran which could selectively identify CORM-2 was designed and it has been successfully applied in living cells, zebrafish and mice. After reacting with CORM-2, both the color and fluorescence signal of the solution are restored, which is ascribed to the reduction of the nitro group. The spectroscopic probe DXPN shows high sensitivity to CORM-2 with a low detection limit of 87 nM and near-infrared fluorescence emission of 712 nm. Notably, this is the first time that paper chips are being used as a carrier to detect CORM-2 through fluorescence signals instead of the traditional liquid phase detection mode of fluorescent probes. These superior properties of the probe make it a promising and reliable tool for exploring the role played by CORM-2 in biological systems. |
Databáze: | OpenAIRE |
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