Influence of mycotoxin zearalenone and its derivatives (alpha and beta zearalenol) on apoptosis and proliferation of cultured granulosa cells from equine ovaries
| Autor: | Maria Elena Dell'Aquila, Francesca Fornelli, A. Giannoccaro, Fiorenza Minervini, Angelo Visconti, P. Minoia |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Fusarium
medicine.medical_specialty endocrine system lcsh:QH471-489 Animal feed Follicular Atresia Alpha (ethology) Apoptosis Food Contamination lcsh:Gynecology and obstetrics Andrology 03 medical and health sciences chemistry.chemical_compound Endocrinology Internal medicine Zeranol medicine lcsh:Reproduction Animals Estrogens Non-Steroidal Horses Mycotoxin Zearalenone lcsh:RG1-991 Cells Cultured 030304 developmental biology 0303 health sciences Granulosa Cells biology Follicular atresia Research 030302 biochemistry & molecular biology Obstetrics and Gynecology food and beverages DNA biology.organism_classification Flow Cytometry Animal Feed In vitro Reproductive Medicine chemistry Female Cell Division Developmental Biology |
| Zdroj: | Reproductive Biology and Endocrinology Reproductive Biology and Endocrinology, Vol 4, Iss 1, p 62 (2006) Reproductive biology and endocrinology 4 (2006): 62–71. info:cnr-pdr/source/autori:Minervini F., Giannoccaro A., Fornelli F., DellAquila M.E., Minoia P., Visconti A./titolo:Influence of mycotoxin zearalenone and its derivatives (alpha and beta zearalenol) on apoptosis and proliferation of cultured granulosa cells from equine ovaries/doi:/rivista:Reproductive biology and endocrinology/anno:2006/pagina_da:62/pagina_a:71/intervallo_pagine:62–71/volume:4 |
| ISSN: | 1477-7827 |
| Popis: | Background The mycotoxin zearalenone (ZEA) and its derivatives, alpha and beta-zearalenol (alpha and beta-ZOL), synthesized by genera Fusarium, often occur as contaminants in cereal grains and animal feeds. The importance of ZEA on reproductive disorders is well known in domestic animals species, particularly in swine and cattle. In the horse, limited data are available to date on the influence of dietary exposure to ZEA on reproductive health and on its in vitro effects on reproductive cells. The aim of this study was to evaluate the effects of ZEA and its derivatives, alpha and beta-ZOL, on granulosa cells (GCs) from the ovaries of cycling mares. Methods The cell proliferation was evaluated by using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test after 3 days exposure at different concentrations of ZEA and its derivatives (from 1 × 10-7 to 0.1 microM). The apoptosis induction was evaluated after 1 day exposure, by DNA analysis using flow cytometry. Results An increase in cell proliferation with respect to the control was observed in the presence of ZEA at 1 × 10-3 and 1 × 10-4 microM and apoptosis was induced by all mycotoxins at different concentrations. Conclusion The simultaneous presence of apoptosis and proliferation in GC cultures treated with zearalenones could indicate that these mycotoxins could be effective in inducing follicular atresia. These effects of zearalenones may result from both direct interaction with oestrogen-receptors as well as interaction with the enzymes 3alpha (beta)-hydroxysteroid dehydrogenase (HSD), involved in the synthesis and metabolism of endogenous steroid hormones. These cellular disturbances, described for the first time in equine GCs cultured in vitro, could be hypothesized as referred to reproductive failures of unknown ethiology in the mare. |
| Databáze: | OpenAIRE |
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