Protective role of α2-macroglobulin against jaw osteoradionecrosis in a preclinical rat model
| Autor: | Xueying Chen, Xiangbo Kong, Jie Li, Wanzhen Zhong, Yang Liu, Fang Silian, Jieyu Chen, Ping Yin |
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| Rok vydání: | 2018 |
| Předmět: |
Male
Cancer Research Pathology medicine.medical_specialty Necrosis Osteoradionecrosis medicine.medical_treatment Radiation-Protective Agents Injections Intralesional medicine.disease_cause Pathology and Forensic Medicine Rats Sprague-Dawley Superoxide dismutase 03 medical and health sciences 0302 clinical medicine Fibrosis medicine Animals Radiotherapy biology Superoxide Dismutase business.industry 030206 dentistry medicine.disease Pregnancy-Associated alpha 2-Macroglobulins Radiation therapy Disease Models Animal Oxidative Stress medicine.anatomical_structure Otorhinolaryngology 030220 oncology & carcinogenesis biology.protein Periodontics Histopathology Cortical bone Oral Surgery medicine.symptom Reactive Oxygen Species business Jaw Diseases Oxidative stress |
| Zdroj: | Journal of Oral Pathology & Medicine. 48:166-173 |
| ISSN: | 0904-2512 |
| DOI: | 10.1111/jop.12809 |
| Popis: | OBJECTIVE We have previously demonstrated the effect of alpha-2-macroglobulin (α2M) in the remediation of radiation-induced cellular damage. Here, we investigated the protective effects of α2M in a preclinical rat model of jaw osteoradionecrosis (ORN). METHODS Eighteen rats were divided randomly into three groups: the control group, the radiation therapy (RT) alone group, and the radiated mandibles pretreated with α2M (α2M + RT) group. One month after radiation, all left molar teeth were extracted. After another 3 months, the animals were sacrificed and body weight, histopathology, microcomputed tomography and immunofluorescence were evaluated in all groups. RESULTS The RT group showed serious alopecia, bone exposure, inflammation, necrosis, fibrosis, and the absence of new bone formation within the socket. The α2M + RT group exhibited less alopecia than the RT group and slight inflammation and fibrosis in the bone marrow cavity. The cortical bone was similar to normal bone tissue. Interestingly, compared with RT group, serum superoxide dismutase levels in the α2M + RT group increased at the 1th day (P = 0.037), 14th day (P = 0.012), while reactive oxygen species levels clearly decreased at the 1th day (P< 0.001), 14th day (P = 0.007), and 28th day (P = 0.013). CONCLUSIONS A clinically translational model of jaw ORN was successfully established and the application of α2M prior to radiation protected the bone from being injured by the radiation, possibly related to oxidative stress. |
| Databáze: | OpenAIRE |
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