A chemically defined medium for rabbit embryo cryopreservation

Autor: Anne Baudot, Gérard Louis, P. Guérin, Samuel Buff, Pierre Bruyère, Loris Commin, Thierry Joly, Elodie Pillet, Anne Josson-Schramme
Přispěvatelé: VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Interactions Cellules Environnement - UR (ICE), Université de Paris - UFR Sciences Fondamentales et Biomédicales [Sciences], Université de Paris (UP), Laboratoire de Recherche Vasculaire Translationnelle (LVTS (UMR_S_1148 / U1148)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP)-Université Sorbonne Paris Nord, ISARA-Lyon
Jazyk: angličtina
Rok vydání: 2013
Předmět:
Serum
Embryology
[SDV.BIO]Life Sciences [q-bio]/Biotechnology
lcsh:Medicine
Cryopreservation
chemistry.chemical_compound
0302 clinical medicine
Cryoprotective Agents
Embryo cryopreservation
Enthalpy
Pregnancy
Small Animals
lcsh:Science
030219 obstetrics & reproductive medicine
Multidisciplinary
Physics
Classical Mechanics
Embryo
04 agricultural and veterinary sciences
Animal Models
Embryo transfer
Solutions
Veterinary Diseases
Thermodynamics
Female
lipids (amino acids
peptides
and proteins)
Rabbits
Research Article
Animal Types
Biology
[SDV.BDLR.RS]Life Sciences [q-bio]/Reproductive Biology/Sexual reproduction
03 medical and health sciences
Cryobiology
Model Organisms
In vivo
Animals
Tissue Survival
Veterinary Prion Diseases
Chromatography
[SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health
Dimethyl sulfoxide
lcsh:R
0402 animal and dairy science
Veterinary Virology
Embryo
Mammalian
040201 dairy & animal science
Chemically defined medium
chemistry
Immunology
Veterinary Science
lcsh:Q
Developmental Biology
Zdroj: PLoS ONE, Vol 8, Iss 8, p e71547 (2013)
PLoS ONE
PLoS ONE, Public Library of Science, 2013, 8 (8), pp.e71547. ⟨10.1371/journal.pone.0071547⟩
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0071547⟩
Popis: International audience; This study evaluates a new synthetic substitute (CRYO3, Ref. 5617, Stem Alpha, France) for animal-based products in rabbit embryo cryopreservation solutions. This evaluation was performed using two approaches: a thermodynamic approach using differential scanning calorimetry and a biological approach using rabbit embryo slow-freezing. During the experiment, foetal calf serum (FCS) was used as a reference. Because FCS varies widely by supplier, three different FCS were selected for the thermodynamic approach. The rabbit embryo slow-freezing solutions were made from Dulbecco's phosphate buffer saline containing 1.5 M Dimethyl Sulfoxide and 18% (v.v−1) of CRYO3 or 18% (v.v−1) of FCS. These solutions were evaluated using four characteristics: the end of melting temperature, the enthalpy of crystallisation (thermodynamic approach) and the embryo survival rates after culture and embryo transfer (biological approach). In the thermodynamic approach, the solutions containing one of the three different FCS had similar mean thermodynamic characteristics but had different variabilities in the overall data with aberrant values. The solution containing CRYO3 had similar thermodynamic properties when compared to those containing FCS. Moreover, no aberrant value was measured in the solution containing CRYO3. This solution appears to be more stable than the solutions containing a FCS. In the biological approach, the in vitro embryo survival rates obtained with the solution containing CRYO3 (73.7% and 81.3%) and with the solution containing a FCS (77.6% and 71.9%) were similar (p = 0.7). Nevertheless, during the in vivo evaluation, the implantation rate (21.8%) and the live-foetuses rate (18.8%) of the CRYO3 group were significantly higher than the implantation rate (7.1%, p = 0.0002) and the live-foetuses rate (5.3%, p = 0.0002) of the FCS group. The pregnancy rate was also higher in the CRYO3 group compared to the FCS group (81.3% and 43.8%, respectively, p = 0.066). We conclude that CRYO3 can be used as a chemically defined substitute for animal-based products in rabbit embryo cryopreservation solutions.
Databáze: OpenAIRE
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