A suppressor of yeast spp81/ded1 mutations encodes a very similar putative ATP-dependent RNA helicase
| Autor: | D. J. Jamieson, J. D. Beggs |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Cell Survival Mutant Genes Fungal Molecular Sequence Data Restriction Mapping Locus (genetics) Saccharomyces cerevisiae Biology Microbiology DEAD-box RNA Helicases Plasmid Adenosine Triphosphate Amino Acid Sequence Cloning Molecular Genes Suppressor Molecular Biology Gene Genetics Base Sequence Nucleic acid sequence RNA RNA Nucleotidyltransferases RNA Helicase A Molecular biology Blotting Southern RNA splicing Mutation RNA Helicases |
| Zdroj: | Molecular microbiology. 5(4) |
| ISSN: | 0950-382X |
| Popis: | The spp81/ded1 mutations were isolated as suppressors of a Saccharomyces cerevisiae pre-mRNA splicing mutation, prp8-1. The SPP81/DED1 gene encodes a putative ATP-dependent RNA helicase. While attempting to clone the wild-type SPP81/DED1 gene we isolated plasmids which were able to suppress the cold-sensitive growth defect of spp81 mutants. These plasmids encoded a gene (named DBP1) which mapped to chromosome XVI and not to the SPP81/DED1 locus on chromosome XV. The cloned gene suppressed the defect of spp81/ded1 mutants when present on both high and low copy-number plasmids but complemented spp81/ded1 null mutants only when present on high copy-number plasmids. In contrast to the SPP81/DED1 gene the DBP1 gene was not essential for cell viability. The nucleotide sequence of the DBP1 gene revealed that it also encoded a putative ATP-dependent RNA helicase which showed considerable similarity at the amino acid level to the SPP81/DED1 protein. |
| Databáze: | OpenAIRE |
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