Transtracheal Administration of Interleukin-12 Induces Neutrophil Responses in the Murine Lung
| Autor: | John R. Gordon, Zsuzsanna Papp, Dorothy M. Middleton, Maria E. Baca-Estrada, Marianna Foldvari, Christine M. Rontved |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Neutrophils
Immunology Respiratory Mucosa In Vitro Techniques Mice Virology medicine Animals Lung Administration Intranasal Mice Inbred BALB C Liposome medicine.diagnostic_test business.industry Chemotaxis Cell Biology medicine.disease Interleukin-12 In vitro Mice Inbred C57BL Pulmonary Alveoli Trachea Chemotaxis Leukocyte medicine.anatomical_structure Bronchoalveolar lavage Liposomes Interleukin 12 Female Nasal administration business Bronchoalveolar Lavage Fluid Infiltration (medical) Respiratory tract |
| Zdroj: | Journal of Interferon & Cytokine Research. 20:191-196 |
| ISSN: | 1557-7465 1079-9907 |
| DOI: | 10.1089/107999000312603 |
| Popis: | Although the roles of interleukin-12 (IL-12) in the immunomodulation of antigen-specific responses are well characterized, the effects of IL-12 on the respiratory tract following mucosal administration are not well defined. Therefore, we investigated changes in the murine lung shortly after intranasal (i.n.) administration of murine IL-12. We showed that IL-12 induced neutrophil influx to the murine lung in both C57BL/6 and BALB/c mice. Histologic examination revealed that intranasal administration of IL-12 with liposomes induced focal neutrophil infiltration into the alveoli and a significant increase in neutrophils in bronchoalveolar lavage fluids when compared with administration of liposomes alone. In vitro chemotaxis assays indicated that the observed pulmonary neutrophil response induced by IL-12 could have been due in part to the direct chemotactic activity of IL-12 for murine neutrophils. |
| Databáze: | OpenAIRE |
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