Effect of the sterilization method on the properties of Bombyx mori silk fibroin films
| Autor: | Dietmar W. Hutmacher, Grant Edwards, Audra M. A. Shadforth, Karina A. George, Peter W. Madden, Sally-Anne Stephenson, Damien G. Harkin, Matthieu J. Laurent, Traian V. Chirila |
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| Rok vydání: | 2013 |
| Předmět: |
Materials science
Surface Properties Tensile properties Cell Culture Techniques 111301 Ophthalmology Silk fibroin Fibroin Biocompatible Materials Bioengineering Cell Line Biomaterials Degradation Tissue engineering Bombyx mori Tensile Strength Cornea Ultimate tensile strength medicine Animals Humans Thin film Composite material Tissue Engineering biology fungi Sterilization Cell adhesion Sterilization (microbiology) Bombyx biology.organism_classification Membrane medicine.anatomical_structure Mechanics of Materials 090301 Biomaterials Fibroins |
| Zdroj: | Materials Science and Engineering C |
| ISSN: | 0928-4931 |
| DOI: | 10.1016/j.msec.2012.10.016 |
| Popis: | We have compared the effects of different sterilization techniques on the properties of Bombyx mori silk fibroin thin films with the view to subsequent use for corneal tissue engineering. The transparency, tensile properties, corneal epithelial cell attachment and degradation of the films were used to evaluate the suitability of certain sterilization techniques including gamma-irradiation (in air or nitrogen), steam treatment and immersion in aqueous ethanol. The investigations showed that gamma-irradiation, performed either in air or in a nitrogen atmosphere, did not significantly alter the properties of films. The films sterilized by gamma-irradiation or by immersion in ethanol had a transparency greater than 98% and tensile properties comparable to human cornea and amniotic membrane, the materials of choice in the reconstruction of ocular surface. Although steam-sterilization produced stronger, stiffer films, they were less transparent, and cell attachment was affected by the variable topography of these films. It was concluded that gamma-irradiation should be considered to be the most suitable method for the sterilization of silk fibroin films, however, the treatment with ethanol is also an acceptable method. |
| Databáze: | OpenAIRE |
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