Generation of Complete Multi-Cell Type Lung Organoids From Human Embryonic and Patient-Specific Induced Pluripotent Stem Cells for Infectious Disease Modeling and Therapeutics Validation

Autor: Rachael N. McVicar, Alicia M. Winquist, Evan Y. Snyder, Sandra L Leibel, Walter D. Niles
Rok vydání: 2020
Předmět:
0301 basic medicine
Patient-Specific Modeling
ciliated cells
Cellular differentiation
lung organoid
Human Embryonic Stem Cells
Cell Culture Techniques
smooth muscle
0302 clinical medicine
Protocol
human pluripotent stem cells
Induced pluripotent stem cell
Lung
Respiratory Tract Infections
pulmonary disease modeling
Cell Differentiation
General Medicine
differentiation
respiratory system
Cell biology
Organoids
medicine.anatomical_structure
Endoderm
Ls30
Coronavirus Infections
Cell type
surfactant
Induced Pluripotent Stem Cells
Pneumonia
Viral
Biology
Models
Biological
Time-Lapse Imaging
03 medical and health sciences
Betacoronavirus
medicine
Humans
Ls40
Progenitor cell
endoderm
Pandemics
lung development
SARS-CoV-2
Mesenchymal stem cell
COVID-19
type 2 alveolar cells
Cell Biology
SARS‐CoV‐2 viral infection
Embryonic stem cell
Ls90
030104 developmental biology
Cell culture
030217 neurology & neurosurgery
Developmental Biology
type 1 alveolar cells
Zdroj: Current Protocols in Stem Cell Biology
ISSN: 1938-8969
Popis: The normal development of the pulmonary system is critical to transitioning from placental‐dependent fetal life to alveolar‐dependent newborn life. Human lung development and disease have been difficult to study due to the lack of an in vitro model system containing cells from the large airways and distal alveolus. This article describes a system that allows human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) to differentiate and form three‐dimensional (3D) structures that emulate the development, cytoarchitecture, and function of the lung (“organoids”), containing epithelial and mesenchymal cell populations, and including the production of surfactant and presence of ciliated cells. The organoids can also be invested with mesoderm derivatives, differentiated from the same human pluripotent stem cells, such as alveolar macrophages and vasculature. Such lung organoids may be used to study the impact of environmental modifiers and perturbagens (toxins, microbial or viral pathogens, alterations in microbiome) or the efficacy and safety of drugs, biologics, and gene transfer. © 2020 Wiley Periodicals LLC. Basic Protocol: hESC/hiPSC dissection, definitive endoderm formation, and lung progenitor cell induction
Databáze: OpenAIRE
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