Macrophage migration inhibitory factor in lung tissue of idiopathic pulmonary fibrosis patients
| Autor: | Elena Bargagli, Ilaria Campo, Simona Inghilleri, Marcella Cintorino, Paola Rottoli, Carmela Olivieri |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Adult
Male 0301 basic medicine Pulmonary and Respiratory Medicine Pathology medicine.medical_specialty Pulmonary Fibrosis Alveolar Epithelium Clinical Biochemistry Disease Lung Disorder 03 medical and health sciences Idiopathic pulmonary fibrosis Usual interstitial pneumonia Fibrosis medicine Humans Lung Macrophage Migration-Inhibitory Factors Molecular Biology Aged Aged 80 and over lung tissue business.industry idiopathic pulmonary fibrosis macrophage migration inhibitory factor Middle Aged respiratory system medicine.disease respiratory tract diseases 030104 developmental biology Cryptogenic Organizing Pneumonia Case-Control Studies Immunohistochemistry Female Macrophage migration inhibitory factor business |
| Popis: | Idiopathic pulmonary fibrosis (IPF) is a severe interstitial lung disorder characterized by a pattern of Usual Interstitial Pneumonia where the presence of fibroblastic foci is the hallmark of the disease.In the present study, we analyzed the migration inhibitory factor (MIF) expression in lung tissue of IPF patients compared with healthy controls and organizing pneumonia (OP) patients focusing into MIF potential role in fibroblastic foci development.The immunohistochemical analysis was performed in 10 IPF patients (7 male), 3 OP patients (2 male), and 3 healthy controls (all male) using the streptavidin-biotin method (Dako).In IPF samples, MIF resulted overexpressed in the areas of active fibrosis and, in particular, in the alveolar epithelium, bronchiolar epithelium, and in the peripheral zones of fibroblastic foci. Bronchiolar epithelium from organizing pneumonia patients resulted only weakly positive for MIF while no evidence of MIF expression was reported for alveolar epithelium. In the control subject group, MIF was unexpressed except for a weak presence in the bronchiolar epithelium.In conclusion, MIF is a pleiotropic cytokine involved in the pathogenesis of IPF being mainly expressed in the areas of remodeling and active fibrosis, in bronchiolar and alveolar epithelium, and in the peripheral zone of fibroblastic foci. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|