Effects of oral administration of (L)-arginine, (L)-NAME and allopurinol on intestinal ischemia/reperfusion injury in rats
| Autor: | Eleftherios V. Margaritis, Ioannis S. Vlachos, Nikolaos Liarakos, P Korkolopoulou, Maria Kostakis, Amalia E. Yanni, George Agrogiannis, Despina Perrea, Antonios Papachristodoulou, Alkisti Pantopoulou, Efstratios Patsouris, Alkiviadis Kostakis |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Male
Time Factors Arginine Allopurinol Administration Oral Nitric Oxide Synthase Type II Pharmacology General Biochemistry Genetics and Molecular Biology Nitric oxide chemistry.chemical_compound Oral administration medicine Animals General Pharmacology Toxicology and Pharmaceutics Rats Wistar Nitrites Peroxidase biology business.industry Nitrotyrosine General Medicine medicine.disease Catalase Rats Nitric oxide synthase Intestines NG-Nitroarginine Methyl Ester chemistry Myeloperoxidase Anesthesia Reperfusion Injury biology.protein Tyrosine business Reperfusion injury medicine.drug |
| Zdroj: | Life sciences. 88(23-24) |
| ISSN: | 1879-0631 |
| Popis: | Aims Intestinal ischemia/reperfusion (I/R) injury is implicated in many clinical conditions, and it performs a fundamental role in their pathophysiologies. Oral administration of antioxidants and nitric oxide (NO) donors ameliorate intestinal injury. Here, the effects of l -arginine, allopurinol and N G -nitro- l -arginine methyl ester ( l -NAME) were investigated. Main methods One hundred twenty-eight male Wistar rats were separated into 4 groups and subjected to occlusion of the superior mesenteric artery for 60 min. The Control group did not receive any substance before the surgical operation. However, the 3 other groups received the following: l -arginine (800 mg/kg body weight; l -Arg group), l -NAME (50 mg/kg; l -NAME group) or allopurinol (100 mg/kg; Allo group). Each substance was given by mouth in 3 equal doses 24, 12 and 1 h before the surgical operation. Each group was then divided into 4 subgroups, which underwent different durations of reperfusion (0, 1, 8 or 24 h). At the end of each time point, blood and tissue samples were collected, and histological examinations were performed. Serum nitrite and catalase, intestinal tissue myeloperoxidase (MPO), inducible nitric oxide synthase (iNOS) and nitrotyrosine (NT) levels were determined. Key findings At each reperfusion time point, the Allo group exhibited the mildest histological lesions in contrast to the l -NAME group, which showed the most severe lesions. MPO was decreased significantly in the Allo and l -Arg groups during reperfusion, and allopurinol administration caused earlier and stronger effect. iNOS and NT levels were higher in the l -Arg group and lower in the Allo group. Serum nitrite and catalase were increased in the l -NAME group after 24 h. Significance Oral administration of allopurinol exerted a strong and protective effect on the intestinal tissue that was subjected to I/R earlier than l -arginine. This finding was also supported with the MPO, iNOS and NT data. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect