Molecular characterization of a bipartite double-stranded RNA virus and its satellite-like RNA co-infecting the phytopathogenic fungus Sclerotinia sclerotiorum
| Autor: | Daohong Jiang, Lijiang Liu, Qihua Wang, Jiatao Xie, Yanping Fu, Jiasen Cheng |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Microbiology (medical)
dsRNA mycovirus botybirnavirus Botrytis porri biology viruses Sclerotinia sclerotiorum lcsh:QR1-502 RNA Botrytis porri RNA virus 1 RNA virus biology.organism_classification RNA virus 1 Microbiology Virology lcsh:Microbiology Virus RNA silencing satellite-like RNA Mycovirus Double-stranded RNA viruses Growth hormone receptor binding Original Research |
| Zdroj: | Frontiers in Microbiology Frontiers in Microbiology, Vol 6 (2015) |
| ISSN: | 1664-302X |
| DOI: | 10.3389/fmicb.2015.00406 |
| Popis: | A variety of mycoviruses have been found in Sclerotinia sclerotiorum. In this study, we report a novel mycovirus Sclerotinia sclerotiorum botybirnavirus 1 (SsBRV1) that was originally isolated from the hypovirulent strain SCH941 of S. sclerotiorum. SsBRV1 has rigid spherical virions that are ~38 nm in diameter, and three dsRNA segments (dsRNA1, 2 and 3 with lengths of 6.4, 6.0 and 1.7 kbp, respectively) were packaged in the virions. dsRNA1 encodes a cap-pol fusion protein, and dsRNA2 encodes a polyprotein with unknown functions but contributes to the formation of virus particles. The dsRNA3 is dispensable and may be a satellite-like RNA (SatlRNA) of SsBRV1. Although phylogenetic analysis of the RdRp domain demonstrated that SsBRV1 is related to Botrytis porri RNA virus 1 (BpRV1) and Ustilago maydis dsRNA virus-H1 (UmV-H1), the structure proteins of SsBRV1 do not have any significant sequence similarities with other known viral proteins with the exception of those of BpRV1. SsBRV1 carrying dsRNA3 seems to have no obvious effects on the colony morphology, but can significantly reduce the growth rate and virulence of S. sclerotiorum. Notably, a growth hormone receptor binding domain (GHBP, Pfam12772) is detected in ORF2-encoded protein of SsBRV1, which have not been reported in any other viruses. These findings provide new insights into the virus taxonomy, virus evolution and the interactions between SsBRV1 and the fungal hosts. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|