Correction of Cronic Liver Failure by Transplantation of Liver Cells Suspension and Cell-engineering Designs (experimental investigation)
| Autor: | S V, Got'e, M Iu, Shagidulin, N A, Onishchenko, M E, Krasheninnikov, I M, Il'inskiĭ, N P, Mozheĭko, A V, Liundup, E A, Volkova, K I, Petrakov, P V, Avramov, N V, Perova, V I, Sevast'ianov |
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| Rok vydání: | 2013 |
| Předmět: |
Male
Pathology medicine.medical_specialty Stromal cell Cell Transplantation Cell CCL4 End Stage Liver Disease Cell therapy Suspensions Animals Medicine Rats Wistar Cell Engineering business.industry Mesenchymal stem cell Liver failure General Medicine Rats Transplantation Disease Models Animal medicine.anatomical_structure Immunology Hepatocytes Bone marrow business Follow-Up Studies |
| Zdroj: | Annals of the Russian academy of medical sciences. 68:44-51 |
| ISSN: | 0869-6047 |
| DOI: | 10.15690/vramn.v68i4.610 |
| Popis: | On an experimental model of chronic fibrotic liver damage (male rats Wistar (n-60), damage of CCl4, the duration of the experiment 90 days) it was studied the effectiveness of cell therapy for the correction of chronic liver failure. These rats were divided into 3 experimental groups: in the Ist-group (control, n=10) isotonic saline (650 mkl.) was injected; in the IInd-group (n=20) suspension of liver cells was applicated in a dose 8 - l0 x 10(6) cells; in the IIIrd-group (n=30) suspension of liver cells and bone marrow cells (mesenchymal stromal cells) in ratio 5:1 were used as cell associates on microparticles intjectable heterogeneous biopolymer hydrogel "SpheroGEL" (cell-engineering design) in common dose 8 - l0 x 10(6) It was ascertained that in the 2nd and in the 3rd groups the accelerated normalization of disturbed liver functional indices (ALT, AST, ALP) took place - to 30 days, but in the control group only to 90 days. The reliable differences in rats ofnormalization offunctional indices were absent between the IInd and the IIIrd groups. But in 90 days by using special histological dyeing it was found out that defibrotic processes in liver tissue were more expressed in the IIIrd group in comparison with the IIIrd group. Received results were consequence of prolonged vital activity of cells (liver cells and mesenchymal stromal bone marrow cells) into cell-engineering designs, which were transplanted in the IIIrd group. The obtained effect can be explained by that the developed cell-engineering designs provide adequate conditions for prolonged vital activity of the transplanted cells. |
| Databáze: | OpenAIRE |
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