Generation of a Murine Monoclonal Antibody Specific forN-Glycolylneuraminic Acid-Containing Gangliosides That Also Recognizes Sulfated Glycolipids
| Autor: | C. Alvarez, Ana María Vázquez, Rolando Pérez, O. Barroso, K.-A. Karlsson, J. Zeuthen, Mauro Alfonso, Luis E. Fernández, Enrique Rengifo, María E. Lanio, B. Lanne, Adriana Carr |
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| Rok vydání: | 1995 |
| Předmět: |
medicine.drug_class
Molecular Sequence Data Immunology Biology Monoclonal antibody Mice chemistry.chemical_compound Glycolipid Antigen Antibody Specificity N-Glycolylneuraminic acid Gangliosides Genetics medicine Animals Humans Mice Inbred BALB C Liposome Sulfoglycosphingolipids Ganglioside Antibodies Monoclonal Immunohistochemistry Molecular biology Carbohydrate Sequence chemistry Biochemistry Organ Specificity Neuraminic Acids Glycolipids Immunostaining |
| Zdroj: | Hybridoma. 14:551-556 |
| ISSN: | 0272-457X |
| DOI: | 10.1089/hyb.1995.14.551 |
| Popis: | The P3 murine monoclonal antibody (MAb) was generated by immunizing BALB/c mice with NeuGcGM3 included into liposomes. The specificity of this MAb was defined by an enzyme-linked immunosorbent assay and immunostaining on thin-layer chromatograms. P3 MAb binds to NeuGc-containing gangliosides and was shown also to react with sulfated glycolipids. A preliminary immunohistochemical study showed that the P3 MAb was able to recognize antigens expressed in human breast tumors. |
| Databáze: | OpenAIRE |
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