Characterization of the lytic-lysogenic switch of the lactococcal bacteriophage Tuc2009
| Autor: | Kenny, JG, Leach, S, de la Hoz, AB, Venema, G, Kok, J, Fitzgerald, GF, Nauta, A, Alonso, JC, van Sinderen, D, Kenny, John G., Hoz, Ana B. de la, Fitzgerald, Gerald F., Alonso, Juan C. |
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| Přispěvatelé: | Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology, Faculty of Science and Engineering |
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Gene Expression Regulation
Viral LACTOBACILLUS-CASEI Inverted repeat ALPHA-HELIX Virus Integration viruses Cloning vector CLONING VECTORS Replication Origin Genome Viral Siphoviridae LACTIC-ACID BACTERIA GENETIC SWITCH Bacteriophage Viral Proteins TRANSCRIPTIONAL ANALYSIS Intergenic region Virology Lysogenic cycle phage Cl Direct repeat DNA binding DNA looping Lysogeny Genetics biology IDENTIFICATION Lactococcus lactis CI REPRESSOR CI biology.organism_classification LAMBDA Repressor Proteins Lytic cycle Cro DNA Viral PHAGE TP901-1 Genes Switch |
| Zdroj: | Virology, 347(2), 434-446. ACADEMIC PRESS INC ELSEVIER SCIENCE |
| ISSN: | 0042-6822 |
| Popis: | Tuc2009 is a temperate bacteriophage of Lactococcus lactis subsp. cremoris UC509 which encodes a CI- and Cro-type lysogenic-lytic switch region. A helix-swap of the 0 helices of the closely related Cl-type proteins from the lactococcal phages r1t and Tuc2009 revealed the crucial elements involved in DNA recognition while also pointing to conserved functional properties of phage Cl proteins infecting different hosts. CI-type proteins have been shown to bind to specific sequences located in the intergenic switch region, but to date, no detailed binding studies have been performed on lactococcal Cro analogues. Experiments shown here demonstrate alternative binding sites for these two proteins of Tuc2009. CI2009 binds to three inverted repeats, two within the intergenic region and one within the cro(2009) gene. This DNA-binding pattern appears to be conserved among repressors of lactococcal and streptococcal phages. The Cro(2009) protein appears to bind to three direct repeats within the intergenic region causing distortion of the bound DNA. (c) 2005 Elsevier Inc. All rights reserved. |
| Databáze: | OpenAIRE |
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