Preparation and characterization of new challenge stocks of SIVmac32H J5 following rapid serial passage of virus in vivo
| Autor: | J. Lines, T. Corcoran, R. Sangster, E.J. Stott, Stuart J. Brown, Stephen Norley, S. MacManus, G. Davis, Neil Almond, J. Cowie, R. Hull, P. Silvera, A. Wade-Evans, A. Jenkins, Richard Stebbings, W. Elsley, M. Sethi, Deborah Ferguson, Neil Berry |
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| Rok vydání: | 2007 |
| Předmět: |
Time Factors
Lymphoid Tissue Molecular Sequence Data Simian Acquired Immunodeficiency Syndrome Spleen Biology Antibodies Viral Virus Replication medicine.disease_cause Virus Serial passage In vivo Cell Line Tumor medicine Animals Humans Amino Acid Sequence Serial Passage General Veterinary Gene Products env Viral Load Simian immunodeficiency virus Virology Macaca fascicularis medicine.anatomical_structure Viral replication Cell culture Simian Immunodeficiency Virus Animal Science and Zoology Sequence Alignment Ex vivo |
| Zdroj: | Journal of Medical Primatology. 36:131-142 |
| ISSN: | 1600-0684 0047-2565 |
| DOI: | 10.1111/j.1600-0684.2007.00224.x |
| Popis: | Background A new challenge stock of the simian immunodeficiency virus SIVmacJ5 has been produced following passage in vivo. Methods SIVmacJ5 3/92 (J5M), was passaged serially through cynomolgus macaques (Macaca fascicularis) by intravenous inoculation of infected spleen cells isolated and prepared 14 days post-infection. Two challenge stocks, SIVmacJ5 S61MLN and SIVmacJ5 S62spl, were prepared by culture of lymphoid tissue ex vivo. Results These virus stocks appeared better adapted for replication in M. fascicularis as demonstrated by a greater persistence of recoverable live virus from the periphery and increased pathology in lymphoid tissues 20 weeks post-challenge as detected by immunohistochemistry. Sequence analysis of the envelope gene from these stocks did not identify marked diversification of sequence as a result of this procedure. Conclusions These stocks display more robust peripheral persistence and tissue pathology in cynomolgus macaques and should prove valuable analysing recombinant vaccines based upon SIVmacJ5 transgenes. |
| Databáze: | OpenAIRE |
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