Genome-wide Identification and Characterization of FCS-Like Zinc Finger (FLZ) Family Genes in Maize (Zea mays) and Functional Analysis of ZmFLZ25 in Plant Abscisic Acid Response
Autor: | Caiji Gao, Chao Yang, Chen Shunquan, Chuanliang Liu, Tang Xiaoyan, Xibao Li, Wei Yan |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
0106 biological sciences
0301 basic medicine Biology maize 01 natural sciences Genome Catalysis lcsh:Chemistry Inorganic Chemistry 03 medical and health sciences chemistry.chemical_compound Arabidopsis Arabidopsis thaliana Physical and Theoretical Chemistry lcsh:QH301-705.5 Molecular Biology Abscisic acid Gene Spectroscopy Zinc finger Functional analysis Organic Chemistry fungi food and beverages General Medicine biology.organism_classification Subcellular localization FLZ-domain protein abiotic stresses ZmFLZ Computer Science Applications Cell biology 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 chemistry ABA signaling 010606 plant biology & botany ZmKIN10 |
Zdroj: | International Journal of Molecular Sciences Volume 22 Issue 7 International Journal of Molecular Sciences, Vol 22, Iss 3529, p 3529 (2021) |
ISSN: | 1422-0067 |
DOI: | 10.3390/ijms22073529 |
Popis: | FCS-like zinc finger family proteins (FLZs), a class of plant-specific scaffold of SnRK1 complex, are involved in the regulation of various aspects of plant growth and stress responses. Most information of FLZ family genes was obtained from the studies in Arabidopsis thaliana, whereas little is known about the potential functions of FLZs in crop plants. In this study, 37 maize FLZ (ZmFLZ) genes were identified to be asymmetrically distributed on 10 chromosomes and can be divided into three subfamilies. Protein interaction and subcellular localization assays demonstrated that eight typical ZmFLZs interacted and partially co-localized with ZmKIN10, the catalytic α-subunit of the SnRK1 complex in maize leaf mesophyll cells. Expression profile analysis revealed that several ZmFLZs were differentially expressed across various tissues and actively responded to diverse abiotic stresses. In addition, ectopic overexpression of ZmFLZ25 in Arabidopsis conferred hypersensitivity to exogenous abscisic acid (ABA) and triggered higher expression of ABA-induced genes, pointing to the positive regulatory role of ZmFLZ25 in plant ABA signaling, a scenario further evidenced by the interactions between ZmFLZ25 and ABA receptors. In summary, these data provide the most comprehensive information on FLZ family genes in maize, and shed light on the biological function of ZmFLZ25 in plant ABA signaling. |
Databáze: | OpenAIRE |
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