Preparation of N, N, N-trimethyl chitosan-functionalized retinoic acid-loaded lipid nanoparticles for enhanced drug delivery to glioblastoma
| Autor: | Pei-Li Zhang, Jian-Li Liu, Junlin Zhou, Lingyan Zhang, Bin Liu, Jie Li |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Sonication Retinoic acid Pharmaceutical Science Lipid nanoparticles Trimethyl chitosan Retinoic acid Glioma Anti-cancer Cytotoxicity Apoptosis 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Cell killing chemistry Biochemistry Apoptosis Solid lipid nanoparticle Drug delivery Biophysics Pharmacology (medical) Nanocarriers Cytotoxicity |
| Zdroj: | Tropical Journal of Pharmaceutical Research; Vol 16, No 8 (2017); 1765-1772 |
| ISSN: | 1596-5996 1596-9827 |
| Popis: | Purpose: To formulate trimethyl chitosan-functionalized retinoic acid-encapsulated solid lipid nanoparticles for the effective treatment of glioma. Methods: Retinoic acid-loaded solid lipid nanoparticles (R-SLNs) were prepared using homogenization followed by sonication. R-SLN surfaces were functionalized electrostatically with trimethyl chitosan as a nanocarrier (TR-SLNs) with enhanced anti-cancer activity. They were evaluated by dynamic light scattering (DLS), scanning electron microscopy, in vitro drug release, and cell cytotoxicity and apoptosis studies. Results: Morphological images showed spherical and uniformly dispersed nanoparticles. A sustained monophasic release pattern was observed throughout the study period. Furthermore, the anti-cancer effect of TR-SLNs was demonstrated by increased cell killing activity compared with the free drug ( p < 0.01); negligible cytotoxicity was observed with blank carriers. Apoptosis assay showed increased cell populations in early/late apoptotic and necrotic phases. Conclusion: This study showed the potential application of surface-modified solid lipid nanoparticles for the effective treatment of brain cancer. Keywords: Lipid nanoparticles, Trimethyl chitosan, Retinoic acid, Glioma, Anti-cancer, Cytotoxicity, Apoptosis |
| Databáze: | OpenAIRE |
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