Unsaturated Fatty Acids Mobilize Intracellular Calcium Independent of IP3Generation and VIA Insertion at the Plasma Membrane
| Autor: | Angelo Benedetti, Rosella Fulceri, Alessandra Gamberucci, Fyfe L. Bygrave |
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| Rok vydání: | 1997 |
| Předmět: |
Male
Thapsigargin Membrane Fluidity Inositol Phosphates Biophysics Biology Biochemistry Calcium in biology Mice chemistry.chemical_compound Free fatty acid receptor 1 Membrane fluidity Animals Carcinoma Ehrlich Tumor Molecular Biology Phospholipase C Sphingosine Cell Membrane Neoplasms Experimental Cell Biology chemistry Second messenger system Fatty Acids Unsaturated Calcium Leukemia Erythroblastic Acute Intracellular |
| Zdroj: | Biochemical and Biophysical Research Communications. 241:312-316 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.1997.7813 |
| Popis: | Addition of oleic and arachidonic acids to Ehrlich ascites tumor cells mobilizes Ca2+ from the same intracellular pool as that mobilized by thapsigargin. Such mobilization occurs in the presence of the phospholipase C inhibitor U73122 as well as in cells treated with pertussis toxin. Co-addition of fatty acids and thapsigargin leads to initial rates of Ca2+ mobilization much greater than that induced by either compound alone. The responses induced by the fatty acids are observed also with other lipophiles like sphingosine, bromo-palmitate and the Ca2+ influx inhibitor econazole; all responses are rapidly reversed by addition of bovine serum albumin. Many of the above effects of fatty acids are observed also in Jurkat T lymphocytes and Friend erythroleukemia cells. The experiments provide evidence of lipid-induced plasma membrane perturbations that influence intracellular Ca2+ mobilization independent of the generation of currently known second messengers. |
| Databáze: | OpenAIRE |
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