Ocular inflammation stimulated by the immunomodulator AS101 [ammonium trichloro(dioxyethelene-O-O') tellurate]
| Autor: | Naofumi Hikita, Kourosh Dastgheib, Devora Sredni, Benjamin Sredni, Robert B. Nussenblatt, Chi-Chao Chan, Michael Albeck |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Pathology
medicine.medical_specialty Eye Diseases genetic structures Eye disease Cell Inflammation Pharmacology Iridocyclitis Major histocompatibility complex Injections Cellular and Molecular Neuroscience Adjuvants Immunologic Anterior Eye Segment Immunopathology medicine Animals Interleukin 6 biology Interleukin-6 business.industry Cell adhesion molecule Histocompatibility Antigens Class II Histology Ethylenes Intercellular Adhesion Molecule-1 medicine.disease eye diseases Sensory Systems Rats Vitreous Body Ophthalmology medicine.anatomical_structure Rats Inbred Lew biology.protein Female sense organs medicine.symptom business |
| Zdroj: | Current Eye Research. 13:603-610 |
| ISSN: | 1460-2202 0271-3683 |
| DOI: | 10.3109/02713689408999894 |
| Popis: | The purpose of this study was to investigate the effect of a novel immunomodulator, AS101 [ammonium trichloro(dioxyethelene-O-O') tellurate], in the eye. Lewis rats were injected intravitreally with AS101 at a concentration of 13 micrograms/ml in one eye and BSS in the contralateral eye. Control animals were injected with BSS into the central vitreous of both eyes. Ocular inflammation was evaluated at 20 hours by histology, immunopathology, and by cell count, protein and cytokine measurement in the aqueous humor. At 20 hours, eyes injected with AS101 developed iridocyclitis and mild vitritis versus minimal inflammation and/or protein in contralateral eyes or eyes of control animals (p = 0.0121). The inflammatory infiltrate was mixed in character. Major Histocompatibility Complex (MHC) class II antigens and intercellular adhesion molecules (ICAM-1) were expressed in the anterior segment of eyes injected with AS101. In the aqueous humor of these eyes there were significant quantities of inflammatory cells, protein (mean +/- SEM = 11.2 +/- 2.3 mg/ml) and the cytokine interleukin 6 (IL-6) (450 units/ml) compared with contralateral eyes (p = 0.0005 for inflammatory cells; protein, mean +/- SEM = 1.6 +/- 0.17 mg/ml; IL-6 = 12 units/ml) and both eyes of control animals injected with BSS (p = 0.8955 for inflammatory cells; protein, OD = 1.5 mg/ml, OS = 0.7 mg/ml; IL-6, OD = 8 units/ml, OS = 13 units/ml). AS101 has a local inflammatory effect in the eye. This compound may activate ocular inflammation by releasing cytokines such as IL-6. |
| Databáze: | OpenAIRE |
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