A novel method of caenophidian snake sex identification using molecular markers based on two gametologous genes
| Autor: | Surin Peyachoknagul, Nararat Laopichienpong, Sunutcha Suntrarachun, Attachai Kantachumpoo, Lawan Chanhome, Panupong Tawichasri, Rattanin Phatcharakullawarawat, Narongrit Muangmai, Kornsorn Srikulnath, Worapong Singchat, Kazumi Matsubara |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Genetics snake Ecology Pcr cloning Single band Sexing Biology gametolog 010603 evolutionary biology 01 natural sciences 03 medical and health sciences 030104 developmental biology Evolutionary biology WAC Small species Identification (biology) CTNNB1 Primer (molecular biology) sex chromosome Gene Ecology Evolution Behavior and Systematics Nature and Landscape Conservation Original Research |
| Zdroj: | Ecology and Evolution |
| ISSN: | 2045-7758 |
| Popis: | Sex identification provides important information for ecological and evolutionary studies, as well as benefiting snake conservation management. Traditional methods such as cloacal probing or cloacal popping are counterproductive for sex identification concerning very small species, resulting in difficulties in the management of their breeding programs. In this study, the nucleotide sequences of gametologous genes (CTNNB1 and WAC genes) were used for the development of molecular sexing markers in caenophidian snakes. Two candidate markers were developed with the two primer sets, and successfully amplified by a single band on the agarose gel in male (ZZ) and two bands, differing in fragment sizes, in female (ZW) of 16 caenophidian snakes for CTNNB1 and 12 caenophidian snakes for WAC. Another candidate marker was developed with the primer set to amplify the specific sequence for CTNNB1W homolog, and the PCR products were successfully obtained in a female‐specific 250‐bp DNA bands. The three candidate PCR sexing markers provide a simple sex identification method based on the amplification of gametologous genes, and they can be used to facilitate effective caenophidian snake conservation and management programs. |
| Databáze: | OpenAIRE |
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