Plasma miRNA Profiles in Pregnant Women Predict Infant Outcomes following Prenatal Alcohol Exposure
| Autor: | Rajesh C. Miranda, Christina D. Chambers, Natalya Zymak-Zakutnya, Jordan J. Schafer, Sridevi Balaraman, Lyubov Yevtushok, Alexander M. Tseng, Wladimir Wertelecki |
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| Přispěvatelé: | Ryabinin, Andrey E |
| Rok vydání: | 2016 |
| Předmět: |
Male
Pathology Maternal Health Biochemistry Families Alcohol Use and Health Pregnancy Infant Mortality Smoking Habits Medicine Young adult lcsh:Science Children Obstetrics Prognosis 3. Good health Nucleic acids Blood Fetal Alcohol Spectrum Disorders Prenatal Exposure Delayed Effects Predictive value of tests Physical Sciences Fetal Alcohol Syndrome Ukraine Statistics (Mathematics) Cohort study medicine.medical_specialty Alcohol Drinking Intellectual and Developmental Disabilities (IDD) Sensitivity and Specificity 03 medical and health sciences Clinical Research Preterm Genetics Humans Statistical Methods Non-coding RNA Socioeconomic status Behavior lcsh:R Infant medicine.disease 030104 developmental biology lcsh:Q Population Groupings Gene expression Mathematics Biomarkers 0301 basic medicine Physiology lcsh:Medicine Reproductive health and childbirth Low Birth Weight and Health of the Newborn Cohort Studies Habits Mathematical and Statistical Techniques Blood plasma Intellectual disability Medicine and Health Sciences Pediatric screening and diagnosis Alcohol Consumption Multidisciplinary Reverse Transcriptase Polymerase Chain Reaction Substance Abuse Obstetrics and Gynecology Hematology Body Fluids Perinatal Care Alcoholism Detection Female Analysis of variance Anatomy Infants Research Article Biotechnology Adult General Science & Technology Research and Analysis Methods Blood Plasma Young Adult Predictive Value of Tests Animals Conditions Affecting the Embryonic and Fetal Periods Nutrition Analysis of Variance Biology and life sciences business.industry Infant Newborn Perinatal Period - Conditions Originating in Perinatal Period Newborn Gene regulation Diet Brain Disorders 4.1 Discovery and preclinical testing of markers and technologies MicroRNAs Age Groups People and Places RNA Women's Health business |
| Zdroj: | PloS one, vol 11, iss 11 PLoS ONE PLoS ONE, Vol 11, Iss 11, p e0165081 (2016) |
| ISSN: | 1932-6203 |
| Popis: | Fetal alcohol spectrum disorders (FASD) are difficult to diagnose since many heavily exposed infants, at risk for intellectual disability, do not exhibit craniofacial dysmorphology or growth deficits. Consequently, there is a need for biomarkers that predict disability. In both animal models and human studies, alcohol exposure during pregnancy resulted in significant alterations in circulating microRNAs (miRNAs) in maternal blood. In the current study, we asked if changes in plasma miRNAs in alcohol-exposed pregnant mothers, either alone or in conjunction with other clinical variables, could predict infant outcomes. Sixty-eight pregnant women at two perinatal care clinics in western Ukraine were recruited into the study. Detailed health and alcohol consumption histories, and 2nd and 3rd trimester blood samples were obtained. Birth cohort infants were assessed by a geneticist and classified as unexposed (UE), heavily prenatally exposed and affected (HEa) or heavily exposed but apparently unaffected (HEua). MiRNAs were assessed in plasma samples using qRT-PCR arrays. ANOVA models identified 11 miRNAs that were all significantly elevated in maternal plasma from the HEa group relative to HEua and UE groups. In a random forest analysis classification model, a combination of high variance miRNAs, smoking history and socioeconomic status classified membership in HEa and UE groups, with a misclassification rate of 13%. The RFA model also classified 17% of the HEua group as UE-like, whereas 83% were HEa-like, at least at one stage of pregnancy. Collectively our data indicate that maternal plasma miRNAs predict infant outcomes, and may be useful to classify difficult-to-diagnose FASD subpopulations. |
| Databáze: | OpenAIRE |
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