Diurnal efficacy of alpha-lipoic acid/nifedipine/glimepiride combination mitigates diabetic neuropathies in rats
| Autor: | Michael Ikechukwu Oraebosi, Temidayo Olutoyin Olurishe, Sherifat Bola Anafi, M. Bisalla |
|---|---|
| Rok vydání: | 2022 |
| Předmět: |
Pharmacology
Meal Antioxidant Nifedipine Thioctic Acid business.industry Alpha-Lipoic Acid medicine.medical_treatment Pharmaceutical Science medicine.disease_cause Antioxidants Diabetes Mellitus Experimental Rats Glimepiride Sulfonylurea Compounds Diabetic Neuropathies medicine Animals Circadian rhythm business Oxidative stress Morning medicine.drug |
| Zdroj: | Annales Pharmaceutiques Françaises. 80:291-300 |
| ISSN: | 0003-4509 |
| Popis: | Summary Objectives: Time-dependent effects of alpha-lipoic acid/nifedipine/glimepiride combination on diabetic neuropathies were investigated in rats. 7 groups (N=9) of rats were used. Materials and methods: First and second groups were apparently normal and diabetic rats respectively, and were administered 1 mL/kg distilled water. The rest of the groups were diabetic and administered 10 mg/kg glimepiride at night-time (8:00 pm). Groups 4-7 were administered additional 20 mg/kg nifedipine at morning-time (8:00 am), while groups 5-7 were also administered 100 mg/kg alpha-lipoic acid (ALA) in the morning, afternoon and night-time respectively (8:00 am, 2:00 pm and 8:00 pm). During the 28 days of oral treatment, paw pressure, tail immersion and motor coordination tests were conducted. The rats were euthanized on the 29th day after a charcoal meal. The small intestines were excised to determine intestinal transit while the brain was collected, homogenised and used to determine levels of oxidative stress. Results: Data show that treatment with ALA at 8:00 am or 2:00 pm significantly (p ≤ 0.01) produced a delay in the onset and improved prognosis of neuropathies. Treatment with ALA at 8:00 pm prevented manifestation of neuropathies throughout the study with positive antioxidant effects. Conclusion: Time-dependent ALA treatment in combination with nifedipine and glimepiride should be studied in humans with an approximately similar circadian timing. This may provide additional clinical therapeutic options for diabetic neuropathies. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|