Antioxidant profile changes in reproductive tissues of rats treated with nicotine
| Autor: | Adeyombo F. Bolarinwa, IP Oyeyipo, Yinusa Raji |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Antioxidant
medicine.medical_treatment Glutathione reductase testes Pharmacology medicine.disease_cause lcsh:Gynecology and obstetrics Male infertility Lipid peroxidation Nicotine Superoxide dismutase chemistry.chemical_compound medicine lcsh:RG1-991 chemistry.chemical_classification biology business.industry Glutathione peroxidase lipid peroxidation medicine.disease rats Reproductive Medicine chemistry biology.protein Original Article business Oxidative stress medicine.drug nicotine |
| Zdroj: | Journal of Human Reproductive Sciences, Vol 7, Iss 1, Pp 41-46 (2014) Journal of Human Reproductive Sciences |
| ISSN: | 1998-4766 0974-1208 |
| Popis: | Objectives: Nicotine intake has been associated with reduced fertility, although the mechanisms responsible are still unclear. However, oxidative stress has been repeatedly implicated as the leading cause of male infertility. This study was therefore designed to investigate the effects of nicotine administration on testicular oxidant and antioxidant system in male albino rats. Materials and Methods: Forty male rats weighing between 150 and 180 g were divided into five groups and treated orally for 30 days. Group I, which served as the control received 0.2 ml/kg normal saline, Groups II and III received 0.5 mg/kg and 1.0 mg/kg body weight (BW) of nicotine respectively. The fourth and fifth groups were administered with 0.5 mg/kg and 1.0 mg/kg BW of nicotine, but were left untreated for another 30 days. Homogenate of testis and epididymis were assayed for lipid peroxidation and anti-oxidant enzyme. Results: The results show a significant decrease (P < 0.05) in testicular glutathione peroxidase, glutathione reductase, catalase and superoxide dismutase while a significant increase (P < 0.05) was observed in testicular lipid peroxidation and nitric oxide level in both groups when compared with the control. Conclusion: This experiment established that nicotine administration is associated with decreased testicular antioxidant and increase testicular lipid peroxidation, which might be a mechanism by which nicotine induce infertility. |
| Databáze: | OpenAIRE |
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