Proteoglycans involved in bidirectional communication between mast cells and hippocampal neurons
| Autor: | María Pilar Ramírez-Ponce, María Ángeles Montes, Guillermo Alvarez de Toledo, Santiago Balseiro-Gomez, Juan A. Flores, Jorge Acosta, Eva Alés |
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| Přispěvatelé: | Universidad de Sevilla. Departamento de Fisiología Médica y Biofísica, Universidad de Sevilla. Departamento de Bioquímica Médica y Biología Molecular e Inmunología |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Immunology Cell Communication Neurotransmission Hippocampal formation Hippocampus NMDA receptors lcsh:RC346-429 Exocytosis Calcium in biology 03 medical and health sciences Cellular and Molecular Neuroscience chemistry.chemical_compound Ca2+ imaging 0302 clinical medicine Calcium imaging medicine Animals neuro-immune Mast Cells Neurotransmitter Cells Cultured lcsh:Neurology. Diseases of the nervous system Calcium signaling Neurons Research General Neuroscience Neuro-immune Coculture Techniques humanities Rats hippocampal neurotransmission Cell biology 030104 developmental biology medicine.anatomical_structure Animals Newborn Hippocampal neurotransmission nervous system Neurology chemistry proteoglycans Proteoglycans Neuron exocytosis 030217 neurology & neurosurgery |
| Zdroj: | idUS: Depósito de Investigación de la Universidad de Sevilla Universidad de Sevilla (US) Journal of Neuroinflammation, Vol 16, Iss 1, Pp 1-13 (2019) Journal of Neuroinflammation idUS. Depósito de Investigación de la Universidad de Sevilla instname |
| ISSN: | 1742-2094 |
| Popis: | Background: Mast cells (MCs) in the brain can respond to environmental cues and relay signals to neurons that may directly influence neuronal electrical activity, calcium signaling, and neurotransmission. MCs also express receptors for neurotransmitters and consequently can be activated by them. Here, we developed a coculture model of peritoneal MCs, incubated together with dissociated hippocampal neurons for the study of cellular mechanisms involved in the mast cell-neuron interactions. Methods: Calcium imaging was used to simultaneously record changes in intracellular calcium [Ca2+]i in neurons and MCs. To provide insight into the contribution of MCs on neurotransmitter release in rat hippocampal neurons, we used analysis of FM dye release, evoked by a cocktail of mediators from MCs stimulated by heat. Results: Bidirectional communication is set up between MCs and hippocampal neurons. Neuronal depolarization caused intracellular calcium [Ca2+]i oscillations in MCs that produced a quick response in neurons. Furthermore, activation of MCs with antigen or the secretagogue compound 48/80 also resulted in a neuronal [Ca2+]i response. Moreover, local application onto neurons of the MC mediator cocktail elicited Ca2+ transients and a synaptic release associated with FM dye destaining. Neuronal response was partially blocked by D-APV, a N-methyl-D-aspartate receptor (NMDAR) antagonist, and was inhibited when the cocktail was pre-digested with chondroitinase ABC, which induces enzymatic removal of proteoglycans of chondroitin sulfate (CS). Conclusions: MC-hippocampal neuron interaction affects neuronal [Ca2+]i and exocytosis signaling through a NMDAR-dependent mechanism. Spanish Ministry of Economy and Competitivity co-funded by the European Social Fund and the Junta de Andalucía (BIO-209 and BIO-236) |
| Databáze: | OpenAIRE |
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