Bone induction by biomimetic PLGA-(PEG-ASP)n copolymer loaded with a novel synthetic BMP-2-related peptide in vitro and in vivo
| Autor: | Qixin Zheng, Zhixia Duan, Hong-Wei Lu, Daping Quan, Zhen-Yu Lin, Jingfeng Li, Shuhua Yang, Xiaodong Guo |
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| Rok vydání: | 2010 |
| Předmět: |
Male
Bone Regeneration Polymers Polyesters Bone Morphogenetic Protein 2 Pharmaceutical Science Biocompatible Materials macromolecular substances Bone morphogenetic protein 2 Bone and Bones Polyethylene Glycols Random Allocation chemistry.chemical_compound Tissue engineering Biomimetics Osteogenesis In vivo medicine Animals Asparaginase Rats Wistar Bone regeneration Reverse Transcriptase Polymerase Chain Reaction technology industry and agriculture Biomaterial Cell Differentiation Mesenchymal Stem Cells Anatomy Molecular biology Controlled release Glycolates Rats PLGA Durapatite medicine.anatomical_structure chemistry Bone marrow Peptides |
| Zdroj: | Journal of Controlled Release. 144:190-195 |
| ISSN: | 0168-3659 |
| DOI: | 10.1016/j.jconrel.2010.02.016 |
| Popis: | BMP-2 is one of the most important growth factors of bone regeneration. Polylactide-co-glycolic acid (PLGA), which is used as a biodegradable scaffold for delivering therapeutic agents, has been intensively investigated. In previous studies, we synthesized a novel BMP-2-related peptide (designated P24) and found that it could enhance the osteoblastic differentiation of bone marrow stromal cells (BMSCs). The objective of this study was to construct a biomimetic composite by incorporating P24 into a modified PLGA-(PEG-ASP)n copolymer to promote bone formation. In vitro, our results demonstrated that PLGA-(PEG-ASP)n scaffolds were shown to be an efficient system for sustained release of P24. Significantly more BMSCs attached to the P24/PLGA-(PEG-ASP)n and PLGA-(PEG-ASP)n membranes than to PLGA, and the cells in the two groups subsequently proliferated more vigorously than those in the PLGA group. The expression of osteogenic markers in P24/PLGA-(PEG-ASP)n group was stronger than that in the PLGA-(PEG-ASP)n and PLGA groups. Radiographic and histological examination, Western blotting and RT-PCR showed that P24/PLGA-(PEG-ASP)n scaffold could induce more effective ectopic bone formation in vivo, as compared with PLGA-(PEG-ASP)n or gelatin sponge alone. It is concluded that the PLGA-(PEG-ASP)n copolymer is a good P24 carrier and can serve as a good scaffold for controlled release of P24. This novel P24/PLGA-(PEG-ASP)n composite promises to be an excellent biomaterial for inducing bone regeneration. |
| Databáze: | OpenAIRE |
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