Barcoded microbial system for high-resolution object provenance
Autor: | Jason Qian, Lorena Lyon, Zhi-xiang Lu, Han-Ying Jhuang, Kole Sedlack, Pamela A. Silver, Michael H. Baym, Fernando H. Ramírez-Guadiana, Michael Springer, Lior Artzi, David Z. Rudner, Mohammad Arammash, Rocío del Carmen Barajas-Ornelas, Sarah A. Boswell, Christopher P. Mancuso, Victoria Jones, Michael Melfi, Ahmad S. Khalil, Akhila Sonti, Siân V. Owen, Mary E. Pettit, Giyoung Jung |
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Rok vydání: | 2020 |
Předmět: |
DNA
Bacterial Spores Provenance Multidisciplinary Computer science Microbiota Distributed computing High resolution Microbial system Object (computer science) Biocontainment Scalability Environmental Microbiology Key (cryptography) DNA Barcoding Taxonomic CRISPR-Cas Systems DNA Fungal RNA Guide Kinetoplastida Nucleic acid detection |
Zdroj: | Science. 368:1135-1140 |
ISSN: | 1095-9203 0036-8075 |
Popis: | DNA barcodes in small packages Under adverse environmental conditions, some microorganisms form spores that provide robust protection for genetic material. Qian et al. developed a system in which DNA barcodes are encapsulated inside nongerminating microbial spores and can be dispersed on objects or in the environment (see the Perspective by Nivala). These barcoded spores provide a durable, specific marker that can be read out quickly with simple equipment. When applied to soil, the spores can be transferred to and from objects around them, enabling tracking at meter-scale resolution. On plant leaves, the spores are not readily transferred, and the authors demonstrate a potential use for tracking agricultural products. Science , this issue p. 1135 ; see also p. 1058 |
Databáze: | OpenAIRE |
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