Dietary iron regulates hepatic hepcidin 1 and 2 mRNAs in mice
| Autor: | Charles Coudray, Christine Feillet-Coudray, Yves Rayssiguier, Elyett Gueux, Andrzej Mazur, Béatrice Romier, Marc Ruivard, Dominique Bayle |
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| Rok vydání: | 2003 |
| Předmět: |
Male
medicine.medical_specialty Ratón Endocrinology Diabetes and Metabolism Down-Regulation Male mice Mice Inbred Strains Mice Random Allocation Endocrinology Hepcidins Downregulation and upregulation Iron homeostasis Hepcidin Internal medicine Gene expression medicine Animals RNA Messenger Messenger RNA Dietary iron Dose-Response Relationship Drug biology Reverse Transcriptase Polymerase Chain Reaction Body Weight Organ Size Actins Up-Regulation Liver biology.protein Iron Dietary Antimicrobial Cationic Peptides |
| Zdroj: | Metabolism. 52:1229-1231 |
| ISSN: | 0026-0495 |
| Popis: | Recently discovered peptide-hepcidin (Hepc) may be a central player in the communication of iron body stores to the intestinal absorptive cells and thus involved in the maintenance of iron homeostasis. The aim of this study was to determine the effects of the level of dietary iron on Hepc gene expression in the liver. OF1 male mice were fed for 3 weeks either control diet (35 mg iron/kg diet), low-iron diet (1 mg iron/kg diet), or high-iron diet (500 mg iron/kg diet), and Hepc 1 and 2 mRNA abundance in the liver was assessed by reverse transcriptase-polymerase chain reaction (RT-PCR). Results clearly showed that Hepc gene expression is upregulated by high dietary iron and downregulated when the dietary iron level is low. Both Hepc 1 and Hepc 2 expression responds coordinately to dietary iron. This work provides additional evidence of the key role of Hepc in the regulation of iron homeostasis. |
| Databáze: | OpenAIRE |
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